Journal Article10.3727/096368912X638946
Decrease of global methylation improves significantly hepatic differentiation of Ad-MSCs: possible future application for urea detoxification.
Claudine Seeliger,Mihaela Culmes,Lilianna Schyschka,X Yan,X Yan,Georg Damm,Zhong-Jing Wang,Zhong-Jing Wang,Jörg Kleeff,Wolfgang E. Thasler,Jan G. Hengstler,Ulrich Stöckle,Sabrina Ehnert,Andreas K. Nussler,Andreas K. Nussler +14 more
TL;DR: Epigenetic changes in hepatocyte-like cells were induced by 5-azacytidine, which, in combination with other supplements, leads to significantly improved metabolic and enzymatic activities compared to nontreated cells, suggesting that these cells may be suitable for clinical application, especially for treatment of urea cycle disorders.
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About: This article is published in Cell Transplantation. The article was published on 01 Jan 2013. The article focuses on the topics: Hepatocyte growth factor & Cellular differentiation.
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Citations
Five freely circulating miRNAs and bone tissue miRNAs are associated with osteoporotic fractures.
Claudine Seeliger,Katrin Karpinski,Alexander T. Haug,Helen Vester,Andreas Schmitt,Jan S. Bauer,Martijn van Griensven +6 more
TL;DR: An important role for several miRNAs are revealed in patients with osteoporotic patients and it is suggested that they may be used as biomarkers for diagnostic purposes and may be a target for treating bone loss and optimizing fracture healing in osteopOrotic patients.
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5-Azacytidine Improves the Osteogenic Differentiation Potential of Aged Human Adipose-Derived Mesenchymal Stem Cells by DNA Demethylation
X. Yan,Sabrina Ehnert,Mihaela Culmes,Anastasia Bachmann,Claudine Seeliger,Lilianna Schyschka,Zhiyong Wang,Afshin Rahmanian-Schwarz,Ulrich Stöckle,Paul A. De Sousa,Jaroslav Pelisek,Andreas K. Nussler +11 more
TL;DR: The data show, for the first time, a decrease of DNA hydroxymethylation in Ad-MSCs which correlates with donor-age and that treatment with 5-Azacytidine provides an approach which could be used to rejuvenate Ad- MSCs from aged donors.
Induction of active demethylation and 5hmC formation by 5-azacytidine is TET2 dependent and suggests new treatment strategies against hepatocellular carcinoma
Sahar Olsadat Sajadian,Sabrina Ehnert,Haghighat Vakilian,Eirini Koutsouraki,Georg Damm,Daniel Seehofer,Wolfgang E. Thasler,Steven Dooley,Hossein Baharvand,Bence Sipos,Andreas K. Nussler +10 more
TL;DR: This study identified a novel function of 5-azacytidine in promoting a TET-mediated generation of 5hmC suggesting that the availability of5-AZA in cancer cells will have various effects on different epigenetic targets.
Self-assembled 3D spheroids and hollow-fibre bioreactors improve MSC-derived hepatocyte-like cell maturation in vitro
Madalena Cipriano,Nora Freyer,Fanny Knöspel,Nuno G. Oliveira,Rita N. Bárcia,Pedro E. Cruz,Helder Cruz,Matilde Castro,Jorge M. Santos,Katrin Zeilinger,Joana P. Miranda +10 more
TL;DR: 3D models tested improved HLC maturation showing a relevant biotransformation capacity and thus provide more appropriate reliable models for mechanistic studies and more predictive systems for in vitro toxicology applications.
42
Molecular Physiognomies and Applications of Adipose-Derived Stem Cells
F. Uzbas,I. D. May,A. M. Parisi,Shannon Kate Thompson,Abdullah Kaya,Andy D. Perkins,Erdogan Memili +6 more
TL;DR: The objective of this review was to provide an update on the current knowledge of the nature and isolation, molecular and epigenetic determinants of the potency, and applications of adipose-derived stromal/stem cells, as well as challenges and future directions.
42
References
Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement
Massimo Dominici,K. Le Blanc,Ingo Mueller,I. Slaper-Cortenbach,Frank C. Marini,Diane S. Krause,Robert J. Deans,Armand Keating,Darwin J. Prockop,Edwin M. Horwitz +9 more
TL;DR: The Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy proposes minimal criteria to define human MSC, believing this minimal set of standard criteria will foster a more uniform characterization of MSC and facilitate the exchange of data among investigators.
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Mesenchymal stem cells reside in virtually all post-natal organs and tissues
TL;DR: The results suggest that the distribution of MSCs throughout the post-natal organism is related to their existence in a perivascular niche, which has implications for understanding MSC biology, and for clinical and pharmacological purposes.
G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis
Makoto Tachibana,Kenji Sugimoto,Masami Nozaki,Jun Ueda,Tsutomu Ohta,Misao Ohki,Mikiko Fukuda,Naoki Takeda,Hiroyuki Niida,Hiroyuki Kato,Yoichi Shinkai +10 more
TL;DR: The results indicate that euchromatic H3-K9 methylation regulated by G9a is essential for early embryogenesis and is involved in the transcriptional repression of developmental genes.
Induction of functional hepatocyte-like cells from mouse fibroblasts by defined factors
Pengyu Huang,Zhiying He,Zhiying He,Shuyi Ji,Huawang Sun,Dao Xiang,Changcheng Liu,Changcheng Liu,Yi-Ping Hu,Xin Wang,Xin Wang,Lijian Hui +11 more
TL;DR: This study demonstrates the direct induction of functional hepatocyte-like (iHep) cells from mouse tail-tip fibroblasts by transduction of Gata4, Hnf1α and Foxa3, and inactivation of p19Arf and provides a novel strategy to generate functional hepatocytes-like cells for the purpose of liver engineering and regenerative medicine.
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Analysis of repetitive element DNA methylation by MethyLight
Daniel J. Weisenberger,Mihaela Campan,Tiffany I. Long,Myungjin Kim,Christian B. Woods,Emerich S. Fiala,Melanie Ehrlich,Peter W. Laird +7 more
TL;DR: This work reports the development of quantitative MethyLight assays to determine the levels of methylated and unmethylated repeats, namely, Alu and LINE-1 sequences and the centromeric satellite alpha (Satα) and juxtacentromeric satellites 2 (Sat2) DNA sequences.
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