Open AccessJournal Article
Cytotoxic T cells in HIV2 seropositive Gambians. Identification of a virus-specific MHC-restricted peptide epitope.
Frances M. Gotch,S McAdam,Catherine E. M. Allsopp,Awen Gallimore,John Elvin,M. P. Kieny,Adrian V. S. Hill,Andrew J. McMichael,Hilton Whittle +8 more
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TL;DR: HLA-B53-restricted, HIV2 gag-specific CTL did not recognize target cells expressing HIV1 gag proteins, indicating that no cellular cross protection to HIV1 could be expected in this case.
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Abstract: A preliminary study of gag-specific, MHC-restricted CD8+ CTL has been performed in nine Gambian patients infected with HIV2. Such CTL were present in at least 55% of patients in fresh peripheral blood mononuclear cells without the requirement for in vitro restimulation. We have identified a nonamer peptide from HIV2 gag that is recognized by CD8+ HLA-B53 CTL using an amino acid sequence motif predicted from analysis of endogenous peptides eluted from HLA-B53 molecules. This peptide, from an HIV2/SIV conserved sequence, has previously been reported to be recognized by CTL from non-human primates vaccinated with recombinant vaccinia virus expressing the gag protein of SIV or infected with SIV virus. HLA-B53-restricted, HIV2 gag-specific CTL did not recognize target cells expressing HIV1 gag proteins, indicating that no cellular cross protection to HIV1 could be expected in this case.
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Citations
Human immunodeficiency virus type 2
TL;DR: The paper was originally published in Journal of General Virology (2002), 83, 1253–1265 and has been republished here with edits since then.
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Polyfunctional T cell responses are a hallmark of HIV-2 infection
Melody G. Duvall,Melody G. Duvall,Melissa L. Precopio,David A. Ambrozak,Assan Jaye,Andrew J. McMichael,Hilton Whittle,Mario Roederer,Sarah Rowland-Jones,Richard A. Koup +9 more
TL;DR: It is found that HIV‐2‐specific CD4+ and CD8+ T cells are more polyfunctional that those specific for HIV‐1 and that polyfunctional HIV‐ 2‐specific T cells produce more IFN‐γ and TNF‐α on a per‐cell basis than monofunctional T cells.
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•Journal Article
Characterization of the peptide binding motif of a rhesus MHC class I molecule (Mamu-A*01) that binds an immunodominant CTL epitope from simian immunodeficiency virus.
Todd M. Allen,J Sidney,del Guercio Mf,Glickman Rl,Gary L. Lensmeyer,Donald A. Wiebe,Robert DeMars,Pauza Cd,R P Johnson,A Sette,David I. Watkins +10 more
TL;DR: The optimal SIV gag CTL epitope restricted by the rhesus MHC class I molecule Mamu-A*01 is defined and a general peptide binding motif for this molecule that is characterized by a dominant position 3 anchor (proline) is defined.
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Differential narrow focusing of immunodominant human immunodeficiency virus Gag-specific cytotoxic T-Lymphocyte responses in infected African and Caucasoid adults and children
Philip J. R. Goulder,Philip J. R. Goulder,Christian Brander,Ken Annamalai,Nolwandle Mngqundaniso,U. Govender,Yanhua Tang,Suqin He,Kelly E. Hartman,Christopher A. O’Callaghan,Graham S. Ogg,Marcus Altfeld,Eric S. Rosenberg,Huyen Cao,Spyros A. Kalams,Michael G. Hammond,Michael Bunce,Stephen I. Pelton,S. A. Burchett,Kenneth McIntosh,Hoosen M. Coovadia,Bruce D. Walker +21 more
TL;DR: Sensitive enzyme-linked immunospot (elispot) assays have been utilized to identify the dominant Gag-specific CTL epitopes targeted by adults and children infected with clade-B or -C virus and there were distinctive differences between the dominant responses made by Caucasoids and Africans.
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CD8+ cells in human immunodeficiency virus type I pathogenesis: cytolytic and noncytolytic inhibition of viral replication.
Otto O. Yang,Bruce D. Walker +1 more
TL;DR: The role of HIV-l-specific cytotoxic T lymphocytes (CTLs) in viral suppression has been defined in experiments analogous to the functional assays used to study the viral suppression by bulk CD8 + cells.
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