Journal Article10.1038/PROTEX.2017.058
CrY2H-seq interactome screening
Joseph R. Ecker,Shelly A. Trigg,Renee M. Garza,Andrew MacWilliams,Joseph R. Nery,Anna Bartlett,Rosa Castanon,Adeline Goubil,Joseph Feeney,Ronan C. O'Malley,Shao-shan Carol Huang,Zhuzhu Zhang,Mary Galli +12 more
TL;DR: A massively-multiplexed yeast two-hybrid method, CrY2H-seq, is described for deep coverage interactome mapping and large-scale matrix screening can be carried out en masse and interactome data can rapidly be generated.
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About: This article is published in Protocol exchange. The article was published on 28 Jun 2017. The article focuses on the topics: Interactome.
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Citations
CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping
Shelly A. Trigg,Shelly A. Trigg,Renee M. Garza,Andrew MacWilliams,Joseph R. Nery,Anna Bartlett,Rosa Castanon,Adeline Goubil,Joseph Feeney,Ronan C. O'Malley,Ronan C. O'Malley,Shao-shan Carol Huang,Zhuzhu Zhang,Mary Galli,Joseph R. Ecker +14 more
TL;DR: A massively multiplexed yeast two-hybrid method, CrY2H-seq, which uses a Cre recombinase interaction reporter to intracellularly fuse the coding sequences of two interacting proteins and next-generation DNA sequencing to identify these interactions en masse, is presented.
References
Fast gapped-read alignment with Bowtie 2
TL;DR: Bowtie 2 combines the strengths of the full-text minute index with the flexibility and speed of hardware-accelerated dynamic programming algorithms to achieve a combination of high speed, sensitivity and accuracy.
An empirical framework for binary interactome mapping
Kavitha Venkatesan,Kavitha Venkatesan,Jean François Rual,Alexei Vazquez,Alexei Vazquez,Ulrich Stelzl,Irma Lemmens,Tomoko Hirozane-Kishikawa,Tong Hao,Martina Zenkner,Xiaofeng Xin,Kwang-Il Goh,Kwang-Il Goh,Kwang-Il Goh,Muhammed A. Yildirim,Nicolas Simonis,Kathrin Heinzmann,Fana Gebreab,Julie M. Sahalie,Sebiha Cevik,Sebiha Cevik,Christophe Simon,Anne Sophie de Smet,Elizabeth Dann,Alex Smolyar,Arunachalam Vinayagam,Haiyuan Yu,David Szeto,Heather Borick,Heather Borick,Amélie Dricot,Niels Klitgord,Niels Klitgord,Ryan R. Murray,Chenwei Lin,Maciej Lalowski,Jan Timm,Kirstin Rau,Charles Boone,Pascal Braun,Michael E. Cusick,Frederick P. Roth,David E. Hill,Jan Tavernier,Erich E. Wanker,Albert-László Barabási,Albert-László Barabási,Marc Vidal +47 more
TL;DR: The results indicate that high-throughput yeast two-hybrid interactions for human proteins are more precise than literature-curated interactions supported by a single publication, suggesting that HT-Y2H is suitable to map a significant portion of the human interactome.
Three-dimensional reconstruction of protein networks provides insight into human genetic disease.
TL;DR: It is found that in-frame mutations are enriched on the interaction interfaces of proteins associated with the corresponding disorders, and that the disease specificity for different mutations of the same gene can be explained by their location within an interface.
427
Network-Based Comparative Analysis of Arabidopsis Immune Responses to Golovinomyces orontii and Botrytis cinerea Infections.
TL;DR: This work compared the Arabidopsis defense responses evoked by the biotrophic fungus Golovinomyces orontii and the necrotrophic fungus Botrytis cinerea through integrative network analysis and identified two network components in which the genes were heavily involved in defense and development, respectively.