Cross-contamination in the molecular detection of Bartonella from paraffin-embedded tissues.
TL;DR: Unless stringent procedures are established and followed to avoid cross-contamination, the molecular detection of Bartonella spp.
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Abstract: The genus Bartonella comprises a group of gram-negative, fastidious bacteria. Because of diagnostic limitations of culture and serologic testing, polymerase chain reaction (PCR) has become a powerful tool for the detection of Bartonella spp. in blood and tissue samples. However, because many wild and domestic animals harbor Bartonella spp., transfer of Bartonella DNA during sample collection or histologic processing could result in false-positive PCR test results. In this study, we describe evidence of Bartonella DNA dissemination and transfer in the necropsy room and during the subsequent processing of formalin-fixed paraffin-embedded tissues. Bartonella DNA was amplified from different areas of the necropsy room, from the liquid paraffin in the tissue processor, and from different parts of the microtome. Unless stringent procedures are established and followed to avoid cross-contamination, the molecular detection of Bartonella spp. from tissue samples obtained at necropsy or processed in a multispecies histopathology laboratory will not be reliable.
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Bartonellosis: an emerging infectious disease of zoonotic importance to animals and human beings
TL;DR: A review of clinically relevant observations related to Bartonella species as emerging pathogens in veterinary and human medicine to provide a review of clinical relevant observations.
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TL;DR: The higher prevalence of Bartonella sp.
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Evaluation of brain tissue or cerebrospinal fluid with broadly reactive polymerase chain reaction for Ehrlichia, Anaplasma, Spotted Fever Group Rickettsia, Bartonella, and Borrelia species in canine neurological diseases (109 cases).
Renee M. Barber,Qiang Li,Pedro Diniz,Brian F. Porter,Edward B. Breitschwerdt,Mary Kate Claiborne,Adam J. Birkenheuer,Jonathan M. Levine,Gwendolyn J. Levine,Kate Chandler,Patrick J. Kenny,Peter P. Nghiem,S. Wei,Craig E. Greene,Marc Kent,Simon R. Platt,Kimberly A. Greer,Scott J. Schatzberg +17 more
TL;DR: The results of this investigation suggest that microorganisms in the genera Ehrlichia, Anaplasma, Rickettsia, Bartonella, and Borrelia are unlikely to be directly associated with canine MEM in the geographic regions evaluated.
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Identification of Bartonella henselae in 2 Cats With Pyogranulomatous Myocarditis and Diaphragmatic Myositis
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