Journal Article10.1016/J.YMETH.2019.05.003
CRISPR/Cas9-based epigenome editing: An overview of dCas9-based tools with special emphasis on off-target activity.
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TL;DR: Attempts to enhance CRISPR-Cas9 specificity by selection of Cas9 orthologs from various bacterial species and their further refinement by introduction of beneficial mutations are reviewed.
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About: This article is published in Methods. The article was published on 15 Jul 2019. The article focuses on the topics: Epigenome editing & Effector.
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Citations
Versatile detection with CRISPR/Cas system from applications to challenges
TL;DR: In this review, the characteristics of four commonly used Cas effectors are analyzed and detection strategies based on the binding activity and cleavage activity of CRISPR/Cas system are fully discussed.
97
Regulation of gene expression by altered promoter methylation using a CRISPR/Cas9-mediated epigenetic editing system
Jeong Gu Kang,Jin Suk Park,Jin Suk Park,Jeong Heon Ko,Jeong Heon Ko,Yong Sam Kim,Yong Sam Kim +6 more
TL;DR: It is suggested that the proven epigenetic editing methods can selectively modulate epigenetic factors such as DNA methylation and have promise for various applications in epigenetics.
CRISPR/dCas-mediated transcriptional and epigenetic regulation in plants.
TL;DR: The recent advances of CRISPR/dCas-mediated transcriptional activation and repression, and epigenetic modifications are summarized and the future directions and broader applications of the CRISpr/d Cas systems in plants are highlighted.
87
A novel strategy for the diagnosis, prognosis, treatment, and chemoresistance of hepatocellular carcinoma: DNA methylation.
Aimei Liu,Qinghua Wu,Qinghua Wu,Dapeng Peng,Irma Ares,Arturo Anadón,Bernardo Lopez-Torres,María-Rosa Martínez-Larrañaga,Xu Wang,Xu Wang,María-Aránzazu Martínez +10 more
TL;DR: This review elaborates on recent key findings regarding molecular biomarkers for HCC early diagnosis, prognosis, and treatment, providing some insights into the difficulty of treating liver cancer and anticancer drug development.
50
Antagonistic and synergistic epigenetic modulation using orthologous CRISPR/dCas9-based modular system
Goran Josipović,Vanja Tadić,Marija Klasić,Vladimir Zanki,Ivona Bečeheli,Felicia Fei-Lei Chung,Akram Ghantous,Toma Keser,Josip Madunić,Maria Bošković,Gordan Lauc,Zdenko Herceg,Aleksandar Vojta,Vlatka Zoldoš +13 more
TL;DR: A modular and extensible CRISPR/dCas9-based toolbox for epigenetic editing and direct gene regulation is presented and strong and persistent synergistic effect on gene transcription is revealed, up to 30 days following cell transfection, suggesting involvement of epigenetic mechanisms in maintenance of the reactivated state.
References
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
16.2K
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,F. A. Ran,David Benjamin Turitz Cox,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +10 more
- 01 Feb 2013
TL;DR: Two different type II CRISPR/Cas systems are engineered and it is demonstrated that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.
12K
CRISPR provides acquired resistance against viruses in prokaryotes
Rodolphe Barrangou,Christophe Fremaux,Hélène Deveau,Melissa Richards,Patrick Boyaval,Sylvain Moineau,Dennis A. Romero,Philippe Horvath +7 more
TL;DR: It is found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences, and CRISPR provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.
Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells
Ophir Shalem,Ophir Shalem,Neville E. Sanjana,Neville E. Sanjana,Ella Hartenian,Xi-Shun Shi,David A. Scott,David A. Scott,Tarjei S. Mikkelsen,Dirk Heckl,Benjamin L. Ebert,David E. Root,John G. Doench,Feng Zhang,Feng Zhang +14 more
TL;DR: This work shows that lentiviral delivery of a genome-scale CRISPR-Cas9 knockout (GeCKO) library targeting 18,080 genes with 64,751 unique guide sequences enables both negative and positive selection screening in human cells, and observes a high level of consistency between independent guide RNAs targeting the same gene and a high rate of hit confirmation.