CRISPR-based rapid and ultra-sensitive diagnostic test for Mycobacterium tuberculosis

TL;DR: Recent advances in the field of CRISPR-based biosensing technologies are discussed and insights of their potential use in a myriad of applications are highlighted.

TL;DR: A comprehensive review of CRISPR/Cas development can be found in this paper, where the advantages and disadvantages of the technology, recent applications, and future considerations are discussed as well as the major challenges for wider application.

TL;DR: CRISPR/Cas-based methods have burst onto the scene, with the potential to power the pathogen detection field, and its hurdles and promises are introduced.

TL;DR: In this paper , a one-pot CRISPR/Cas13a-based visual biosensor was proposed and developed for the rapid and low-cost nucleic acid detection by combining Cas13a cleavage and Recombinase Polymerase Amplification (RPA) in a onepot reaction in a disposable tube-in-tube vessel, amplicon contamination could be completely avoided.

TL;DR: The 18th global report on tuberculosis (TB) provides a comprehensive and up-to-date assessment of the TB epidemic and of the progress made in TB prevention, care and control at global, regional and country level, using data reported by 197 countries and territories.

TL;DR: It is shown that RNA-guided DNA binding unleashes indiscriminate single-stranded DNA cleavage activity by Cas12a that completely degrades ssDNA molecules, which is also a property of other type V CRISPR-Cas12 enzymes.

TL;DR: LshC2c2 is a RNA-guided RNase which requires the activity of its two HEPN domains, suggesting previously unidentified mechanisms of RNA targeting and degradation by CRISPR systems.

TL;DR: The mechanisms of CRISPR interference and its roles in microbial physiology and evolution are reviewed and potential applications of this novel interference pathway are discussed.