Book Chapter10.1007/978-3-642-59379-6_8
Continuous-Flow and Continuous-Exchange Cell-Free Translation Systems and Reactors
V.A. Shirokov,Peter Nikolayevich Moscow Region Simonenko,Sergey Vladimirovich Biryukov,Alexander S. Spirin +3 more
- 01 Jan 2002
- pp 91-107
12
TL;DR: This work states that gene expression in vitro, in cell-free translation or transcription-translation systems, is an alternative that allows full control and high flexibility of conditions.
read more
Abstract: Progress in biotechnology generates a growing demand for convenient and productive technologies of gene expression on preparative scale. In vivo systems for expression of foreign genes in bacterial or eukaryotic cells are efficient and widely used. However, difficulties associated with cytotoxicity, proteolytic degradation or improper folding and aggregation of synthesized proteins are often encountered in vivo. The in vivo expression systems are restricted by mechanisms of cell control and allow limited opportunities for solving these problems. On the other hand, gene expression in vitro, in cell-free translation or transcription-translation systems, is an alternative that allows full control and high flexibility of conditions.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Exploring the Potential of Cell-Free Protein Synthesis for Extending the Abilities of Biological Systems.
Khushal Khambhati,Gargi Bhattacharjee,Nisarg Gohil,Darren Braddick,Vishwesh V. Kulkarni,Vijai Singh +5 more
TL;DR: The present review highlights the recent advances and uses of CFPS systems in biomedical, therapeutic, and biotechnological applications and highlights possible solutions to the potential biosafety issues that may be encountered while using CFPS technology.
Economic Analysis of Batch and Continuous Biopharmaceutical Antibody Production: a Review
TL;DR: This paper provides a review of the work that has been published in the literature on computational process design and economic analysis methods on continuous biopharmaceutical antibody production and its comparison with a conventional batch process.
Continuous-Exchange Protein-Synthesizing Systems
TL;DR: The basic principles of continuous-exchange protein synthesizing systems, and protocols for Escherichia coli and wheat germ translation and transcription-translation systems are described, and the ways to improve substrate supply in cell-free systems and mRNA design for eukaryotic system are discussed.
54
Cell-free Protein Expression Using the Rapidly Growing Bacterium Vibrio natriegens
Daniel J. Wiegand,Daniel J. Wiegand,Henry H. Lee,Nili Ostrov,George M. Church,George M. Church +5 more
TL;DR: A general protocol is described for the preparation of V. natriegens crude cell extracts and cell-free protein synthesis that can be easily integrated into existing protein synthesis pipelines to facilitate advances in bio-production and synthetic biology applications.
Effective non-viral leader for cap-independent translation in a eukaryotic cell-free system
Lyubov Shaloiko,I.E. Granovsky,Tatiana V. Ivashina,Vladimir N. Ksenzenko,V.A. Shirokov,Alexander S. Spirin +5 more
TL;DR: The obelin mRNA leader is proposed to be used for effective cap-independent translation in eukaryotic cell-free systems, including combined transcription-translation systems with uncontrolled phage polymerase-catalyzed accumulation of mRNA.
25
References
In vitro synthesis of protein in microbial systems.
TL;DR: This review is devoted to studies involving synthesis of complete proteins in cell-free systems, and a number of laboratories have studied the DNA-dependent RNA directed synthesis of both viral and bacterial proteins in E. coli extracts.
759
•Book
Transcription and translation : a Practical Approach
B.D. Hames,Stephen J. Higgins +1 more
- 01 Jan 1984
TL;DR: This work aims to demonstrate the efforts towards in-vitro transcription-translation-translation co-operation in prokaryotic cell-free systems and demonstrates the ability of these systems to co-operate with each other on the basis of individual cell types.
584
Cell-free translation of messenger RNA in a wheat germ system.
Ann H. Erickson,Günter Blobel +1 more
TL;DR: Methods that are used to study the in vitro synthesis of proteins, each comprise several percent of total cellular protein, such as globin, prolactin, the caseins, and to the synthesis of the lysosomal proteins cathepsin D, β-glucuronidase, and glucocerebrosidase are described.
308
Prolonging cell‐free protein synthesis with a novel ATP regeneration system
Dong-Myung Kim,James R. Swartz +1 more
TL;DR: A new approach for the regeneration of adenosine triphosphate (ATP) during cell-free protein synthesis was developed to prolong the synthesis and also to avoid the accumulation of inorganic phosphate, demonstrated in a batch system derived from Escherichia coli.
277