Comprehensive identification of glycated peptides and their glycation motifs in plasma and erythrocytes of control and diabetic subjects.
Qibin Zhang,Matthew E. Monroe,Athena A. Schepmoes,Therese R. W. Clauss,Marina A. Gritsenko,Da Meng,Vladislav A. Petyuk,Richard D. Smith,Thomas O. Metz +8 more
TL;DR: Using immunodepletion, enrichment, and fractionation strategies, this report comprehensively identified glycated proteins in control and diabetic human plasma and erythrocytes and identified 7749 unique glycated peptides, corresponding to 3742 unique proteins.
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Abstract: Non-enzymatic glycation of proteins sets the stage for formation of advanced glycation end-products and development of chronic complications of diabetes. In this report, we extended our previous methods on proteomics analysis of glycated proteins to comprehensively identify glycated proteins in control and diabetic human plasma and erythrocytes. Using immunodepletion, enrichment, and fractionation strategies, we identified 7749 unique glycated peptides, corresponding to 3742 unique glycated proteins. Semi-quantitative comparisons showed that glycation levels of a number of proteins were significantly increased in diabetes and that erythrocyte proteins were more extensively glycated than plasma proteins. A glycation motif analysis revealed that some amino acids were favored more than others in the protein primary structures in the vicinity of the glycation sites in both sample types. The glycated peptides and corresponding proteins reported here provide a foundation for potential identification of novel markers for diabetes, hyperglycemia, and diabetic complications in future studies.
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Citations
Review: Glycation of human serum albumin
Jeanethe Anguizola,Ryan Matsuda,Omar S. Barnaby,K. S. Hoy,Chunling Wa,Erin Debolt,Michelle Koke,David S. Hage +7 more
TL;DR: This review examines work that has been conducted in the study and analysis of glycated HSA and considers the effects of glycation on the binding of HSA with drugs, fatty acids and other solutes and the potential clinical significance of these effects.
380
Carbonic Anhydrases in Metazoan Model Organisms: Molecules, Mechanisms, and Physiology.
Ashok Aspatwar,Martti Tolvanen,Harlan Barker,Leo Syrjänen,Susanna Valanne,Sami Purmonen,Abdul Waheed,William S. Sly,Seppo Parkkila +8 more
TL;DR: Functional studies demonstrate that CAs are not only linked to the regulation of pH homeostasis, the classical role of CAs but also contribute to a plethora of previously undescribed functions.
76
Enhanced oxidative stress and damage in glycated erythrocytes.
Chloé Turpin,Aurélie Catan,Alexis Guerin-Dubourg,Xavier Debussche,Susana B. Bravo,Ezequiel Álvarez,Jean M. H. van den Elsen,Olivier Meilhac,Philippe Rondeau,Emmanuel Bourdon +9 more
TL;DR: The objective was to decipher the impact of glycation on erythrocyte structure, oxidative stress parameters and capacity to interact with cultured human endothelial cells, with a possible relevance to diabetes.
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Probing Protein Glycation by Chromatography and Mass Spectrometry: Analysis of Glycation Adducts.
TL;DR: Although the spectrum of quantitatively assessed AGE structures is continuously increases, application of untargeted profiling techniques for identification of new products is desired, especially for in vivo characterization of anti-glycative systems.
58
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