Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses.

TL;DR: The potential of metagenomics for characterization of the normal viral population in a healthy community and identification of viruses that could pose a threat to humans through zoonosis is discussed and a new model of the Koch's postulates named the ‘Metagenomic Koch's Postulates’ is proposed.

TL;DR: This study provides a powerful culture-independent approach for virus discovery in invertebrates by assembling viral genomes directly from host immune response products without prior virus enrichment or amplification, and proposes that invertebrate viruses discovered by this approach may include previously undescribed human and vertebrate viral pathogens that are transmitted by arthropod vectors.

TL;DR: These examples of synergistic interactions of plant pathogens that lead to disease complexes might prove to be more common than expected and understanding the underlying mechanisms might have important implications in plant disease epidemiology and management.

TL;DR: The key challenge from a routine testing lab perspective will not be one of investment in platforms-which could even be outsourced to commercial sequencing services-but one of having the skills and expertise to analyse the large datasets generated and their subsequent interpretation.

TL;DR: The observation that irradiated combs from affected colonies can be repopulated with naive bees suggests that infection may contribute to colony collapse disorder (CCD).

TL;DR: This work applies the definition of EIDs used in the medical and veterinary fields to botany and highlights a series of emerging plant diseases, including EIDs of cultivated and wild plants, some of which are of significant conservation concern.

TL;DR: SSAKE is a tool for aggressively assembling millions of short nucleotide sequences by progressively searching through a prefix tree for the longest possible overlap between any two sequences to help leverage the information from short sequence reads by stringently assembling them into contiguous sequences that can be used to characterize novel sequencing targets.

TL;DR: The results show the efficiency of this technique in characterizing viral DNA components of several geminiviruses from experimental and natural host plant sources and will considerably accelerate genomics of at least gemini-, nano- and circoviruses in the future.