Complete Genomic Analysis of Enterococcus faecium Heat-Resistant Strain Developed by Two-Step Adaptation Laboratory Evolution Method.
11
TL;DR: The developed heat-resistant BIOPOP-3 ALE strain developed in this study and the two-step ALE method are expected to be widely applied in industrial and academic fields and it is expected that the identified variants which occurred specifically in heat- resistant strain will enhance molecular biological understanding and be broadly applied to the biological engineering field.
read more
Abstract: Stress resistance is an important trait expected of lactic acid bacteria used in food manufacturing. Among the various sources of stress, high temperature is a key factor that interrupts bacterial growth. In this regards, constant efforts are made for the development of heat-resistant strains, but few studies were done accompanying genomic analysis to identify the causal factors of the resistance mechanisms. Furthermore, it is also thought that tolerance to multiple stresses are equally important. Herein, we isolated one Enterococcus faecium strain named BIOPOP-3 and completed a full-length genome sequence. Using this strain, a two-step adaptive laboratory evolution (ALE) method was applied to obtain a heat-resistant strain, BIOPOP-3 ALE. After sequencing the whole genome, we compared the two full-length sequences and identified one non-synonymous variant and four indel variants that could potentially confer heat resistance, which were technically validated by resequencing. We experimentally verified that the evolved strain was significantly enhanced in not only heat resistance but also acid and bile resistance. We demonstrated that the developed heat-resistant strain can be applied in animal feed manufacturing processes. The multi-stress-resistant BIOPOP-3 ALE strain developed in this study and the two-step ALE method are expected to be widely applied in industrial and academic fields. In addition, we expect that the identified variants which occurred specifically in heat-resistant strain will enhance molecular biological understanding and be broadly applied to the biological engineering field.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Metabolic engineering coupled with adaptive evolution strategies for the efficient production of high-quality L-lactic acid by Lactobacillus paracasei.
TL;DR: The main indicators for industrial production of high-quality lactic acid at elevated temperatures are high titer, productivity, yield, and optical purity, and a high optical purity strain developed through the CRISPR-Cas9 gene editing platform is developed.
50
Complete Genome Sequence of the Newly Developed Lactobacillus acidophilus Strain With Improved Thermal Adaptability.
TL;DR: In this paper, a new L. acidophilus strain with improved heat resistance while retaining the existing beneficial properties through the adaptive laboratory evolution (ALE) method was developed, which improved the existing limit of thermal resistance from 65°C to 75°C.
The novel probiotic preparation based on Lactobacillus spp. mixture on the intestinal bacterial community structure of Cherry Valley duck.
Shuiqin Shi,Mengrui Ge,Yan Xiong,Yixun Zhang,Wenhui Li,Zhimuzi Liu,Jianfen Wang,Enhui He,Liming Wang,Du Li Ping-kun Zhou +9 more
1
Genomic and Phenotypic Insight into the Probiotic Potential of Lactic Acid Bacterial spp. Associated with the Human Gut Mucosa
TL;DR: Genome annotation suggested bacterial adaptation to both human physiological and industrial manufacturing–related stressors, and basic genetic and phenotypic information supports their contention as probiotic adjuncts to conventional medical therapy.
1
The novel probiotic preparation based on Lactobacillus spp. mixture on the intestinal bacterial community structure of Cherry Valley Duck
24 Oct 2022
TL;DR: In this article , the results of the antibacterial activity and tolerance tests, the highly active strains Lactobacillus casei 1.2435, L. salivarius L621, and L4 from the intestines of Cherry Valley ducks were selected.
References
Identification of novel anti-inflammatory probiotic strains isolated from pulque
Edgar Torres-Maravilla,Edgar Torres-Maravilla,Marion Lenoir,Marion Lenoir,Lino Mayorga-Reyes,Thibault Allain,Thibault Allain,Harry Sokol,Philippe Langella,Philippe Langella,María Elena Sánchez-Pardo,Luis G. Bermúdez-Humarán,Luis G. Bermúdez-Humarán +12 more
TL;DR: One of the selected strains, Lactobacillus sanfranciscensis LBH1068, improved mice health as observed by a reduction of weight loss, significant decreases in gut permeability, and cytokine modulation.
65
Comparative genomic analysis revealed great plasticity and environmental adaptation of the genomes of Enterococcus faecium.
TL;DR: This study shows that the Enterococcus faecium species has great genomic plasticity and high versatility to occupy broad ecological roles, dwelling as non-harmful dairy and animal gut commensals as well as significant nosocomial pathogens that disseminate antibiotic resistance genes.
Comparative genome analysis reveals key genetic factors associated with probiotic property in Enterococcus faecium strains
Vikas C. Ghattargi,Vikas C. Ghattargi,Meghana A. Gaikwad,Bharati S. Meti,Yogesh Nimonkar,Kunal Dixit,Om Prakash,Yogesh S. Shouche,Shrikant Pawar,Dhiraj P. Dhotre +9 more
TL;DR: Higher genomic stability, absence of known virulence factors and antibiotic resistance genes and close genomic relatedness with marketed probiotics makes E. faecium 17OM39 a potential probiotic candidate.
Enhancing probiotic stability in industrial processes
Miguel Gueimonde,Borja Sánchez +1 more
TL;DR: During recent years, the knowledge acquired on the molecular basis of stress-tolerance of probiotics has increased the authors' understanding on their responses to industrial stresses, and this knowledge on stress-response may nowadays be used for the selection of the best strains and industrial conditions in terms of probiotic stability in the final product.
Related Papers (1)
Zhang Huitu,Xiaonong Wu,Lu Fuping +2 more
- 25 Jan 2019