Journal Article10.1016/J.ARCHORALBIO.2018.05.008
Comparison of two digestion strategies on characteristics and differentiation potential of human dental pulp stem cells.
Maziar Ebrahimi Dastgurdi,Fatemeh Ejeian,Marzie Nematollahi,Ahmad Motaghi,Mohammad Hossein Nasr-Esfahani +4 more
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TL;DR: This study aimed to compare the behavior of dental pulp stem cells (DPSCs) after isolation using solutions containing either collagenase/dispase or collagenase alone to find out which enzyme solutions gave rise to different populations of DPSCs.
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About: This article is published in Archives of Oral Biology. The article was published on 01 Sep 2018. The article focuses on the topics: Dispase & Dental pulp stem cells.
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Citations
Best Practices for Preparing a Single Cell Suspension from Solid Tissues for Flow Cytometry.
Andrew Reichard,Kewal Asosingh +1 more
TL;DR: Evaluation of the single cell suspension is essential before proceeding with the labeling of the cells as high viability and absence of cell debris and aggregates are critical for flow cytometry.
162
Recycle the dental fairy’s package: overview of dental pulp stem cells
TL;DR: The history of dental pulp stem cell study and the detail experimental method including isolation, culture, cryopreservation, and the differentiation strategy to different cell lineage are reviewed.
Enzymatic Isolation, Amplification and Characterization of Dental Pulp Stem Cells
Nela Pilbauerová,Tomáš Soukup,Tereza Suchánková Kleplová,Jan Suchánek +3 more
TL;DR: Enzymatic isolation, amplification and characterization of dental pulp stem cells successfully isolated 10 populations of DPSCs with high vitality and proliferation capacity. The isolated DPSCs highly expressed CD markers and showed a high osteogenic and chondrogenic potential.
Effects and mechanisms of basic fibroblast growth factor on the proliferation and regenerative profiles of cryopreserved dental pulp stem cells.
Lihua Luo,Yanni Zhang,Hongyu Chen,Fengting Hu,Xiaoyan Wang,Zhenjie Xing,Abdullkhaleg Ali Albashari,Jian Xiao,Yan He,Qingsong Ye,Qingsong Ye +10 more
TL;DR: This study aimed to explore the effects of adding recombinant bFGF on DPSCs after 3‐month cryopreservation as well as the underlying mechanisms.
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Regenerative Capacity of Dental Pulp Stem Cells: A Systematic Review.
Adlin Saroja Rosaian,Gururaj Narayana Rao,Sunil Paramel Mohan,Mahalakshmi Vijayarajan,Rebekkah C Prabhakaran,Anand Sherwood +5 more
TL;DR: The application of proposed markers, on a pulpectomized tissue derived from human teeth, may be helpful to determine the regenerative potential and the usefulness in regenerative medicine and tissue engineering.
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References
Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement
Massimo Dominici,K. Le Blanc,Ingo Mueller,I. Slaper-Cortenbach,Frank C. Marini,Diane S. Krause,Robert J. Deans,Armand Keating,Darwin J. Prockop,Edwin M. Horwitz +9 more
TL;DR: The Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy proposes minimal criteria to define human MSC, believing this minimal set of standard criteria will foster a more uniform characterization of MSC and facilitate the exchange of data among investigators.
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Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo
TL;DR: Postnatal human DPSCs are isolated that have the ability to form a dentin/pulp-like complex and are compared with human bone marrow stromal cells, known precursors of osteoblasts.
Mesenchymal stem cells reside in virtually all post-natal organs and tissues
TL;DR: The results suggest that the distribution of MSCs throughout the post-natal organism is related to their existence in a perivascular niche, which has implications for understanding MSC biology, and for clinical and pharmacological purposes.
Stem Cell Properties of Human Dental Pulp Stem Cells
Stan Gronthos,Jaime S. Brahim,W. Li,Larry W. Fisher,Natasha Cherman,Alan Boyde,Pamela DenBesten,P. Gehron Robey,Songtao Shi +8 more
TL;DR: It is demonstrated that DPSCs possess stem-cell-like qualities, including self-renewal capability and multi-lineage differentiation, including single-colony-derived DPSC strains that differ from each other with respect to their rate of odontogenesis.
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Mesenchymal Stem Cells Derived from Dental Tissues vs. Those from Other Sources: Their Biology and Role in Regenerative Medicine
TL;DR: This article will review the isolation and characterization of the properties of different dental MSC-like populations in comparison with those of other MSCs, such as BMMSCs.
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