Comparison of in-vitro outcomes from cryopreserved oocytes and sibling fresh oocytes.
Sandrine Chamayou,Carmelita Alecci,Carmen Ragolia,Giorgia Storaci,E Maglia,E Russo,Antonino Guglielmino +6 more
TL;DR: The significant decrease in meiotic spindle retrieval rate before freezing and after thawing procedures suggests that cryoconservation induces irreversible damage to microtubule repolymerization.
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Abstract: In Italy, the restrictive IVF law generalizes the indication for oocyte freezing for surplus oocytes in 78.5% of in-vitro assisted reproductive cycles. With a view to understanding better what the prospects for intracytoplasmic sperm injection (ICSI) on frozen-thawed oocytes might be, the consequences of freeze-thaw procedures on fertilization, cleavage rates and embryo quality obtained from frozen-thawed oocytes were studied and compared with the results obtained from sibling fresh oocytes. Eleven IVF and 29 ICSI on 76 and 169 fresh oocytes were performed and the corresponding 40 ICSI on 221 sibling frozen-thawed oocytes. There was no difference in terms of fertilization rate between fresh and sibling frozen-thawed oocytes. The cleavage rate (98.0 and 94.4% with fresh oocytes in IVF and ICSI; 77.3% with frozen-thawed oocytes in ICSI; P < 0.001) and embryo quality (grade I embryos over total embryos: 36.7 and 22.2% with fresh oocytes in IVF and ICSI; 12.1% with frozen-thawed oocytes in ICSI; respectively P < 0.001 and P < 0.05) were statistically lower after oocyte cryopreservation. The significant decrease in meiotic spindle retrieval rate before freezing (62.4%) and after thawing procedures (43.4%; P < 0.001) suggests that cryoconservation induces irreversible damage to microtubule repolymerization. The consequences of oocyte cryopreservation procedures on embryo development are reviewed.
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Citations
Oocyte, embryo and blastocyst cryopreservation in ART: systematic review and meta-analysis comparing slow-freezing versus vitrification to produce evidence for the development of global guidance
Laura Rienzi,Clarisa R. Gracia,Roberta Maggiulli,Andrew R. LaBarbera,Daniel J. Kaser,Filippo Maria Ubaldi,Sheryl Vanderpoel,Sheryl Vanderpoel,Catherine Racowsky +8 more
TL;DR: A systematic review and meta-analysis of clinical outcomes following slow-freezing/thawing versus vitrification/warming of oocytes and embryos is provided to inform the development of World Health Organization guidance on the most effective cryopreservation method.
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Over 900 oocyte cryopreservation babies born with no apparent increase in congenital anomalies.
TL;DR: With more live born data accumulating, this procedure may become mainstream as a fertility preservation option, particularly for women diagnosed with malignancy requiring cytotoxic therapy and a registry would help to assure the safest, most expeditious development of this technology.
461
A critical appraisal of cryopreservation (slow cooling versus vitrification) of human oocytes and embryos
TL;DR: In this article, it was shown that slow cooling is associated with lower survival rates and compromised development relative to vitrification when applied to metaphase II oocytes, although the vitrification results have predominantly been obtained using direct contact with liquid nitrogen and there is some evidence that optimal protocols for slow cooling of MII oocytes are yet to be established.
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Age-specific probability of live birth with oocyte cryopreservation: an individual patient data meta-analysis.
TL;DR: The live birth probabilities calculated would enable more accurate counseling and informed decisions for infertile women considering oocyte cryopreservation and suggest that policy makers should consider oocyte freezing as an integral part of prevention and treatment of infertility.
181
Slow freezing and vitrification differentially modify the gene expression profile of human metaphase II oocytes
TL;DR: The low pregnancy rates commonly observed when using human MII oocytes after slow freezing-thawing may be explained by the alterations of the oocyte gene expression profile.
121
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