Comparative mutagenic frequency, efficiency, effectiveness and rate of ethyl methane sulphonate and sodium Azide in groundnut (Arachis hypogaea L.)
TL;DR: In this article , the frequency, effectiveness and relative efficiency of mutagenic treatments in M1 generation were calculated based on the seedling characters and the genotypes showed varied response to different concentrations of the two mutagens.
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Abstract: The present investigation was carried out with an objective of assessing the response of four selected groundnut genotypes viz., Smruti, Devi, ICGV 7220 and ICGV 2266 to doses of mutagenic treatments. Mutagenic concentrations of 0.2%, 0.3% and 0.4% of EMS and sodium azide were taken respectively. The frequency, effectiveness and relative efficiency of mutagenic treatments in M1 generation were calculated based on the seedling characters. The genotypes showed varied response to different concentrations of the two mutagens. SA produced a greater number of mutants with increase in concentration. Smruti showed reduced mean seedling height. Genotype vigour index was high for ICGV 7220. Efficiency of mutagen varied according to genotype. Effectiveness ranged from 1.975 (V3E2) to 8.148 (V1N1). Mutation rate (in terms of efficiency and effectiveness) was highest in SA treated Smruti (7.099) M1 generation compared to EMS (3.447).
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Citations
Studying the Effect of Mutations on some genotypes of Peanut
TL;DR: In this article , four varieties of peanuts were treated with three different concentrations of sodium azide (NaN 3 ); 0.00 control (0.00), 0.01% and 0.03% for inducing mutation and planted in a randomized complete block design.
Callus Induction, Regeneration of Rice Variety BRRI Dhan29 and Determination of Lethal Dose Of Ethyl Methanesulfonate
NA Khan,MI Uddin,JK Ghosh,MH Rashid +3 more
- 21 Nov 2023
TL;DR: Callus induction and regeneration of rice variety BRRI Dhan29 and determination of lethal dose of ethyl methanesulfonate (EMS) for genetic improvement. The study developed a protocol for callus induction and regeneration of BRRI Dhan29 and determined the LD50% of EMS on callus survival.
References
Molecular Mechanisms of Mutations
Ernst Freese
- 01 Jan 1971
TL;DR: In growing or adult cells, most DNA resides in chromosomes inside the cell nucleus, whereas a smaller fraction is found in plastids (mitochondria, chloroplasts, etc.).
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•Journal Article
Mutagenic effects of sodium azide and its application in crop improvement.
Fahad Al-Qurainy,Salim Khan +1 more
105
A deletion common to two independently derived waxy mutations of maize.
TL;DR: The data suggests that EMS is capable of inducing structural alterations in plant genes in addition to the point mutations normally ascribed to EMS-induced mutations.
68
•Journal Article
An assessment of gamma ray induced mutations in rice (oryza sativa l.)
TL;DR: Gamma ray seed treatment (10, 20 and 30 kR doses) of two cultivars, namely, MPRI-7 and Lanjhi was found to affect pollen and spikelet fertility in M1 generation at higher dose.
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