Journal Article10.1186/s13099-023-00570-z
Comparative genomic analysis of Fusobacterium nucleatum reveals high intra-species diversity and cgmlst marker construction
Arslan Dovletgeldiyev,Chen Shen,Kexin Li,Songnian Hu,Zilong He +4 more
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TL;DR: The pan-genome shows a clear open tendency and most of plasmids in Fusobacterium nucleatum are mainly transmitted intraspecifically, and the phylogenetic tree based on cgmlst molecular markers is revealed.
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Abstract: Abstract Background Fusobacterium nucleatum is a one of the most important anaerobic opportunistic pathogens in the oral and intestinal tracts of human and animals. It can cause various diseases such as infections, Lemierre's syndrome, oral cancer and colorectal cancer. The comparative genomic studies on the population genome level, have not been reported. Results We analyzed all publicly available Fusobacterium nucleatum s’ genomic data for a comparative genomic study, focusing on the pan-genomic features, virulence genes, plasmid genomes and developed cgmlst molecular markers. We found the pan-genome shows a clear open tendency and most of plasmids in Fusobacterium nucleatum are mainly transmitted intraspecifically. Conclusions Our comparative analysis of Fusobacterium nucleatum systematically revealed the open pan-genomic features and phylogenetic tree based on cgmlst molecular markers. What’s more, we also identified common plasmid typing among genomes. We hope that our study will provide a theoretical basis for subsequent functional studies.
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Citations
Short-sighted evolution of virulence for invasive gut microbes: From hypothesis to tests
Pauline D. Scanlan,Fernando Baquero,Bruce R. Levin +2 more
TL;DR: This perspective reexamines the short-sighted evolution hypothesis, proposing that invasive gut microbes evolve from commensal bacteria through niche expansion, leading to virulence, and presents a framework for testing this hypothesis using genomic data and animal model experiments.
Case Report: the Use of Metagenomic Next-Generation Sequencing to Diagnose Lemierre’s Syndrome Caused by Lost Root Canal Fillings in a 33-Year-old Patient with Metabolic Syndrome
Yimeng Zhou,Yongzhen Zhai,Yan Wang,Dan Zhang,Guohe Feng +4 more
TL;DR: A 33-year-old patient with metabolic syndrome was diagnosed with Lemierre's syndrome via metagenomic next-generation sequencing, caused by lost root canal fillings, highlighting the importance of this diagnostic tool in undetermined fever cases.
Short-sighted evolution of virulence for invasive gut microbes: from hypothesis to tests.
Pauline D. Scanlan,Fernando Baquero,Bruce R. Levin +2 more
- 24 Jul 2024
TL;DR: This perspective re-examines the short-sighted evolution hypothesis, proposing that invasive gut microbes evolve from commensal bacteria through niche expansion, leading to virulence, and presents a framework for testing this hypothesis using genomic data and animal model experiments.
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