Comparative Analysis of Human Mesenchymal Stem Cells from Bone Marrow, Adipose Tissue, and Umbilical Cord Blood as Sources of Cell Therapy
Hye Jin Jin,Yun Kyung Bae,Mi-Yeon Kim,Soon-Jae Kwon,Hong Bae Jeon,Soo Jin Choi,Seong Who Kim,Yoon Sun Yang,Wonil Oh,Jong Wook Chang +9 more
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TL;DR: It is found that recombinant Ang-1 as potential soluble paracrine factor or its small interference RNA (siRNA) was responsible for this beneficial effect in part by preventing inflammation.
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Abstract: Various source-derived mesenchymal stem cells (MSCs) have been considered for cell therapeutics in incurable diseases. To characterize MSCs from different sources, we compared human bone marrow (BM), adipose tissue (AT), and umbilical cord blood-derived MSCs (UCB-MSCs) for surface antigen expression, differentiation ability, proliferation capacity, clonality, tolerance for aging, and paracrine activity. Although MSCs from different tissues have similar levels of surface antigen expression, immunosuppressive activity, and differentiation ability, UCB-MSCs had the highest rate of cell proliferation and clonality, and significantly lower expression of p53, p21, and p16, well known markers of senescence. Since paracrine action is the main action of MSCs, we examined the anti-inflammatory activity of each MSC under lipopolysaccharide (LPS)-induced inflammation. Co-culture of UCB-MSCs with LPS-treated rat alveolar macrophage, reduced expression of inflammatory cytokines including interleukin-1α (IL-1α), IL-6, and IL-8 via angiopoietin-1 (Ang-1). Using recombinant Ang-1 as potential soluble paracrine factor or its small interference RNA (siRNA), we found that Ang-1 secretion was responsible for this beneficial effect in part by preventing inflammation. Our results demonstrate that primitive UCB-MSCs have biological advantages in comparison to adult sources, making UCB-MSCs a useful model for clinical applications of cell therapy.
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Citations
Adult Stem Cells for Bone Regeneration and Repair
Maria Rosa Iaquinta,Elisa Mazzoni,Ilaria Bononi,John Charles Rotondo,Chiara Mazziotta,Monica Montesi,Simone Sprio,Anna Tampieri,Mauro Tognon,Fernanda Martini +9 more
TL;DR: This review will describe bone structure and the osteogenic differentiation of stem cells, the role of mesenchymal stem cells for bone repair/regrowth in the tissue engineering field and their recent progress in clinical applications will be discussed.
214
Endometrial and Menstrual Blood Mesenchymal Stem/Stromal Cells: Biological Properties and Clinical Application.
Mahmood Bozorgmehr,Mahmood Bozorgmehr,Shanti Gurung,Saeedeh Darzi,Saeedeh Darzi,Shohreh Nikoo,Somaieh Kazemnejad,Amir-Hassan Zarnani,Amir-Hassan Zarnani,Caroline E. Gargett,Caroline E. Gargett +10 more
TL;DR: A wealth of data on the biological properties of eMSC and MenSC has recently been published, and it will be important to address their mechanism of action in preclinical models of human disease to maximize their potential for clinical application.
Exosomes derived from pioglitazone-pretreated MSCs accelerate diabetic wound healing through enhancing angiogenesis
Yiqiang Hu,Ranyang Tao,Lang Chen,Yuan Xiong,Hang Xue,Liangcong Hu,Chenchen Yan,Xudong Xie,Ze Lin,Adriana C. Panayi,Bobin Mi,Guohui Liu +11 more
TL;DR: In this paper, the effect of exosomes derived from mesenchymal stem cells (MSCs) pretreated with pioglitazone (PGZ-Exos) on diabetic wound healing was investigated.
Dielectrophoresis for Biomedical Sciences Applications: A Review.
TL;DR: This paper critically reviews the fundamentals, recent progress, current challenges, future directions and potential applications of research investigations in the medical sciences utilizing DEP technique and will act as a guide and reference for medical researchers and scientists to explore and utilize the DEp technique in their research fields.
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Mesenchymal Stem and Progenitor Cells in Regeneration: Tissue Specificity and Regenerative Potential
TL;DR: This review provides compact information on the role of stem cells, in particular, MSC in regeneration, and shows promise for regenerative cell therapy with a rather limited level of risk.
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