Commonly used methods for extracellular vesicles' enrichment: Implications in downstream analyses and use.
Marta Clos-Sansalvador,Marta Monguió-Tortajada,Santiago Roura,Marcella Franquesa,Francesc E. Borràs +4 more
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TL;DR: In this article , the authors present the most widely used extracellular vesicles (EV) isolation methods, from the initial differential ultracentrifugation (dUC) to newest approaches.
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About: This article is published in European Journal of Cell Biology. The article was published on 01 Apr 2022. and is currently open access. The article focuses on the topics: Medicine & Isolation (microbiology).
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Citations
Extracellular vesicles as tools and targets in therapy for diseases
Mudasir A Kumar,S. K. Baba,Hana Q. Sadida,Sara Al Marzooqi,Jayakumar Jerobin,Faisal Altemani,Naseh Ateahallah Algehainy,Mohammad Alanazi,Abdul-Badi Abou-Samra,Rakesh Kumar,Ammira-Sarah Al-Shabeeb Akil,Muzafar A. Macha,Rashid Mir,Ajaz A. Bhat +13 more
TL;DR: The roles of EVs across various conditions, including cancers, neurodegenerative disorders, diabetes, viral infections, autoimmune and renal diseases, are discussed, emphasizing the potential advancements in molecular diagnostics and drug delivery.
203
Comprehensive isolation of extracellular vesicles and nanoparticles
TL;DR: In this paper , a step-by-step protocol for sequential isolation of large and small extracellular vesicles, nonvesicular fractions, exomeres and supermeres from the same starting material is presented.
Extracellular Vesicle Preparation and Analysis: A State‐of‐the‐Art Review
Zesheng Wang,Xiaoyu Zhou,Qing Kong,Hua He,Jiayu Sun,Wei Qiu,Liang Zhang,Mengsu Yang +7 more
TL;DR: EV preparation and analysis techniques are complex and heterogeneous due to the inherent nature of EVs. This review explores the latest advancements in the field, focusing on microfluidic and non-microfluidic platforms, single EV analysis, and AI-powered approaches.
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MMP-9 as Prognostic Marker for Brain Tumours: A Comparative Study on Serum-Derived Small Extracellular Vesicles
Gabriella Dobra,Edina Gyukity-Sebestyen,Matyas Bukva,Maria Harmati,Valentina Nagy,Zoltán Szabó,Tibor Pankotai,Almos Klekner,Krisztina Buzas +8 more
TL;DR: In this paper , a comparative study was conducted to evaluate the possible additional utility of the MMP-9 level of serum-derived small extracellular vesicles (sEVs) for characterising brain tumours.
Extracellular Vesicles as Novel Drug-Delivery Systems through Intracellular Communications
Yasunari Matsuzaka,Ryu Yashiro +1 more
TL;DR: The possible molecular mechanisms by which miRNAs in exosomes participate in the post-transcriptional regulation of signaling pathways via cell–cell communication as novel DDSs, especially small EVs are outlined.
References
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TL;DR: The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities, and a checklist is provided with summaries of key points.
Shedding light on the cell biology of extracellular vesicles.
TL;DR: Extracellular vesicles are now considered as an additional mechanism for intercellular communication, allowing cells to exchange proteins, lipids and genetic material.
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
TL;DR: This unit describes different approaches for exosome purification from various sources, and discusses methods to evaluate the purity and homogeneity of the purified exosomes preparations.
Biological properties of extracellular vesicles and their physiological functions
María Yáñez-Mó,Pia Siljander,Zoraida Andreu,Apolonija Bedina Zavec,Francesc E. Borràs,Edit I. Buzás,Krisztina Buzas,Krisztina Buzas,Enriqueta Casal,Francesco Cappello,Joana Carvalho,Joana Carvalho,Eva Colas,Anabela Cordeiro da Silva,Anabela Cordeiro da Silva,Stefano Fais,Juan M. Falcón-Pérez,Irene M. Ghobrial,Bernd Giebel,Mario Gimona,Mario Gimona,Michael W. Graner,Ihsan Gursel,Mayda Gursel,Niels H. H. Heegaard,Niels H. H. Heegaard,An Hendrix,Peter Kierulf,Katsutoshi Kokubun,Maja Kosanović,Veronika Kralj-Iglič,Eva-Maria Krämer-Albers,Saara Laitinen,Cecilia Lässer,Thomas Lener,Thomas Lener,Erzsébet Ligeti,Aija Linē,Georg Lipps,Alicia Llorente,Jan Lötvall,Mateja Manček-Keber,Antonio Marcilla,María Mittelbrunn,Irina Nazarenko,Esther N. M. Nolte-‘t Hoen,Tuula A. Nyman,Lorraine O'Driscoll,Mireia Olivan,Carla Oliveira,Carla Oliveira,Éva Pállinger,Hernando A. del Portillo,Hernando A. del Portillo,Jaume Reventós,Jaume Reventós,Marina Rigau,Eva Rohde,Eva Rohde,Marei Sammar,Francisco Sánchez-Madrid,Nuno Santarém,Nuno Santarém,Katharina Schallmoser,Katharina Schallmoser,Marie Stampe Ostenfeld,Willem Stoorvogel,Roman Štukelj,Susanne G. van der Grein,M. Helena Vasconcelos,M. Helena Vasconcelos,Marca H. M. Wauben,Olivier De Wever +72 more
TL;DR: A comprehensive overview of the current understanding of the physiological roles of EVs is provided, drawing on the unique EV expertise of academia-based scientists, clinicians and industry based in 27 European countries, the United States and Australia.
Progress in Exosome Isolation Techniques.
TL;DR: An overview of exosome isolation techniques is provided, opening up new perspectives towards the development more innovative strategies and devices for more time saving, cost effective, and efficient isolations ofExosomes from a wide range of biological matrices.