Journal Article10.1016/S0965-1748(00)00133-8
Cloning and functional expression of a chitinase cDNA from the common cutworm, Spodoptera litura, using a recombinant baculovirus lacking the virus-encoded chitinase gene
59
TL;DR: A Chitinase cDNA named Slchi was cloned from the epidermis of the common cutworm, Spodoptera litura, and the enzymatic properties of its recombinant proteins were characterized, suggesting its involvement in the digestive system for old cuticle.
read more
About: This article is published in Insect Biochemistry and Molecular Biology. The article was published on 27 Apr 2001. The article focuses on the topics: Recombinant virus & Chitinase.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Insect group II chitinase OfChtII promotes chitin degradation during larva-pupa molting.
TL;DR: Investigation of the role of OfChtII during the molting of Ostrinia furnacalis, a model lepidopteran pest insect, suggested an important role for Cht II during insect molting and provided a strategy for the coordinated degradation of cuticular chitin during beetle molting by ChtII, ChtI and Chi‐h.
33
Transcriptional Regulation of a Chitinase Gene by 20-Hydroxyecdysone and Starvation in the Oriental Fruit Fly, Bactrocera dorsalis
TL;DR: Quantitative real-time PCR analyses indicated that BdCht2 was mainly expressed during the larval-pupal and pupal-adult transitions and plays an important role in the molting process of B. dorsalis larvae.
Functional analysis of eight chitinase genes in rice stem borer and their potential application in pest control
TL;DR: The functions of eight SSB chitinase genes are elucidated, found that CsCht10 could be a good candidate for pest control and knocked down each gene at the developmental stage where highest expression was observed.
25
Enhancement of the biological activity of nucleopolyhedrovirus through disruption of the peritrophic matrix of insect larvae by chlorfluazuron
TL;DR: The enhancement of Spodoptera litura (F.) nucleopolyhedrovirus (SlNPV) activity using the chitin synthesis inhibitor chlorfluazuron was investigated and showed that the peritrophic matrices of S. litura exposed to chlor fluazuron alone, or the combination treatment, were markedly disrupted.
23
Purification and Characterization of a Novel Isozyme of Chitinase from Bombyx mori
TL;DR: Kinetic analysis and reaction-pattern analysis indicated that 75-kDa chitinase is an endo- or random-type hydrolytic enzyme to produce the β anomeric product and that it prefers the longer N-acetylchitooligosaccharides, suggesting, together with the N-terminal amino acid sequence, that the 75- kDa ch itinase belongs to family 18 of glycosyl hydrolases.
21
References
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
256.9K
•Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
203K
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
TL;DR: A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original.
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
TL;DR: A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described, providing a pure preparation of undegraded RNA in high yield and can be completed within 4 h.
69.5K
Clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
TL;DR: The sensitivity of the commonly used progressive multiple sequence alignment method has been greatly improved and modifications are incorporated into a new program, CLUSTAL W, which is freely available.