Circulating miRNAs in Type 2 Diabetic Patients with and without Albuminuria in Malaysia
Siti Yazmin Zahari Sham,Chin Tat Ng,S. Azwar,Wai Kien Yip,Maha Abdullah,Kalaiselvam Thevandran,Malina Osman,Heng Fong Seow +7 more
TL;DR: Investigation of differentially expressed miRNAs in the sera of type 2 diabetes mellitus patients with and without albuminuria in a selected Malaysian population found upregulation of previously known miRNA and possibly novel ones, namely, miR-874-3p and miGFR, in the serum of T2DM patients, was found.
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Abstract: Introduction: Diabetic kidney disease (DKD) remains the leading cause of chronic kidney disease. Dysregulation of circulating miRNAs has been reported, suggesting their pathological roles in DKD. This study aimed to investigate differentially expressed miRNAs in the sera of type 2 diabetes mellitus (T2DM) patients with and without albuminuria in a selected Malaysian population. Method: Forty-one T2DM patients on follow-up at a community clinic were divided into normo-(NA), micro-(MIC), and macroalbuminuria (MAC) groups. Differential levels of miRNAs in 12 samples were determined using the pathway-focused (human fibrosis) miScript miRNA qPCR array and was validated in 33 samples, using the miScript custom qPCR array (CMIHS02742) (Qiagen GmbH, Hilden, Germany). Results: Trends of upregulation of 3 miRNAs in the serum, namely, miR-874-3p, miR-101-3p, and miR-145-5p of T2DM patients with MAC compared to those with NA. Statistically significant upregulation of miR-874-3p (p = 0.04) and miR-101-3p (p = 0.01) was seen in validation cohort. Significant negative correlations between the estimated glomerular filtration rate (eGFR) and miR-874-3p (p = 0.05), miR-101-3p (p = 0.03), and miR-145-5p (p = 0.05) as well as positive correlation between miR-874-3p and age (p = 0.03) were shown by Pearson’s correlation coefficient analysis. Conclusion: Upregulation of previously known miRNA, namely, miR-145-5p, and possibly novel ones, namely, miR-874-3p and miR-101-3p in the serum of T2DM patients, was found in this study. There was a significant correlation between the eGFR and these miRNAs. The findings of this study have provided encouraging evidence to further investigate the putative roles of these differentially expressed miRNAs in DKD.
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Citations
From Euglycemia to Recent Onset of Type 2 Diabetes Mellitus: A Proof-of-Concept Study on Circulating microRNA Profiling Reveals Distinct, and Early microRNA Signatures
M. Greco,Maria Mirabelli,Alessandro Salatino,Francesca Accattato,V. Aiello,Francesco Saverio Brunetti,Eusebio Chiefari,Salvatore A. Pullano,Antonino S. Fiorillo,Daniela Foti,Antonio Brunetti +10 more
TL;DR: This cross-sectional study investigates the differential expression patterns of circulating miRNAs in the serum of patients with impaired fasting glucose and new-onset T2D, with respect to euglycemic controls, using a high-throughput 384-well array and real-time PCR to identify novel biomarkers for the early diagnosis of type 2 diabetes mellitus.
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Prediction of mRNA Targets of miR-101-3p in Diabetic Kidney Disease by Bioinformatics Tools
Siti Yazmin Zahari Sham,S. Azwar,Kien Yip Wai,Chin Tat Ng,Maha Abdullah,Kalaiselvam Thevandran,Malina Osman,Fong Seow Heng +7 more
TL;DR: In this paper , the authors used bioinformatics tools to predict the mRNA targets of miR-101-3p for chronic kidney disease (CKD) in type 2 diabetic patients.
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Elucidation of mRNA targets of miR-145-5p in diabetic kidney disease using bioinformatics analysis
Siti Yazmin Zahari Sham,S. Azwar,Kien Yip Wai,Maha Abdullah,Kalaiselvam Thevandran,Malina Osman,Heng Fong Seow +6 more
TL;DR: In this article , the authors reported a trend of upregulation of miR-145-5p in sera of type 2 diabetic patients with macroalbuminuria in a selected Malaysian population, which concurred with previous in vivo and in vitro studies of kidney disease.
A novel miR-99b-5p-Zbp1 pathway in microglia contributes to the pathogenesis of schizophrenia
Lalit Kaurani,Md. Rezaul Islam,Urs Heilbronner,Dennis M. Krüger,Jiayin Zhou,Aditi Methi,Judith Strauss,Ranjit Pradhan,Susanne Burkhardt,Tonatiuh Pena,Lena Erlebach,Anika Bühler,Monika Budde,Fanny Senner,Mojtaba Oraki Kohshour,Eva C. Schulte,Max Schmauss,Eva Z. Reininghaus,Georg Juckel,Deborah Kronenberg-Versteeg,Ivana Delalle,Francesca Odoardi,Alexander Flügel,Thomas G. Schulze,Peter Falkai,Farahnaz Sananbenesi,Andre Fischer +26 more
TL;DR: In this article , the miR-99b-5p-Zbp1 pathway in microglia was found to contribute to the pathogenesis of schizophrenia in both the prefrontal cortex and blood of patients.
MicroRNAs Associated with Chronic Kidney Disease in the General Population and High-Risk Subgroups—A Systematic Review
Dipuo D. Motshwari,Don M Matshazi,Rajiv T Erasmus,Andre Pascal Kengne,Tandi E. Matsha,Cindy George +5 more
TL;DR: In this article , a review aimed to characterize miRNAs associated with CKD and/or measures of kidney function and kidney damage in the general population, and also in high risk subgroups, including people with hypertension (HTN), diabetes mellitus (DM) and human immunodeficiency virus (HIV) infection.
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