Circ_0009910 regulates growth and metastasis and is associated with poor prognosis in gastric cancer.
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TL;DR: It is demonstrated that circ_0009910 acts as a prognostic biomarker and promote cell proliferation, migration, invasion and EMT in GC, indicating thatcirc_000 9910 may be a novel potential biomarkerand therapeutic target of GC.
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Abstract: OBJECTIVE Circular RNAs (circRNAs) have recently shown capabilities as gene regulators in mammals. In this study, we aimed to evaluate the effects and mechanism of circ_0009910 in gastric cancer (GC). PATIENTS AND METHODS Circ_0009910 expression was quantified by Real-time PCR in human GC cell lines and tissues. Association between circ_0009910 levels and clinicopathological factors and patient's prognosis was analyzed. The roles of circ_0009910 in regulating GC cell proliferation, colony formation, migration, and invasion were evaluated in vitro. Western blot analysis was conducted to detect the expressions of molecular markers of epithelial-mesenchymal transition (EMT). RESULTS Circ_0009910 expression level was elevated in GC tissues and cell lines and associated with clinical stage (p = 0.032), distant metastasis (p = 0.028) and differentiation (p = 0.007). Kaplan-Meier survival analysis indicated that circ_0009910 expression in positive group has a worse overall survival compared to the negative group (p = 0.0013). Multivariate analysis showed that circ_0009910 was an independent risk factor for GC (HR = 2.346, 95% CI: 1.673-3.775, p = 0.006). Knockdown of circ_0009910 expression can suppress BGC823 and AGS cells proliferation, migration and invasion in vitro experiments. The results of Western blot indicated that knockdown of circ_0009910 increased expression of E-cadherin and decreased expression of the mesenchymal markers, snail and N-cadherin. CONCLUSIONS Altogether, we demonstrate that circ_0009910 acts as a prognostic biomarker and promote cell proliferation, migration, invasion and EMT in GC, indicating that circ_0009910 may be a novel potential biomarker and therapeutic target of GC.
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Circular RNAs: Promising Molecular Biomarkers of Human Aging-Related Diseases via Functioning as an miRNA Sponge.
TL;DR: The promising circRNAs being disease biomarkers are reviewed, focused on their regulatory function by acting as miRNA sponges, and their roles in cancer, cardiovascular or neurodegenerative diseases, osteoarthritis, rheumatoid arthritis, diabetes, and other human aging-related diseases are described.
Circ_0009910 promotes imatinib resistance through ULK1-induced autophagy by sponging miR-34a-5p in chronic myeloid leukemia
TL;DR: Circ_0009910 accelerated imatinib-resistance in CML cells by modulating ULK1-induced autophagy via targeting miR-34a-5p, providing a potential target in imatinIB resistance of CML.
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Circular RNAs: Emerging Role in Cancer Diagnostics and Therapeutics.
TL;DR: The emerging role of circRNAs as biomarkers and therapeutic targets in cancer is summarized.
Hsa_circ_0009910: oncogenic circular RNA targets microRNA-145 in ovarian cancer cells
Ying Li,Shuang Lin,Na An +2 more
TL;DR: Hsa_circ_0009910, representing unfavorable prognosis, induced proliferative and motile phenotypes by suppressing miR-145 in ovarian cancer cells and was correlated with the poor prognosis of ovarian cancer patients.
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Role of circular RNAs in disease progression and diagnosis of cancers: An overview of recent advanced insights.
Safir Ullah Khan,Munir Ullah Khan,Muhammad Imran Khan Almani,Fadahunsi Adeola Abraham,Asad Khan,Shuang Gao,Muhammad Bilal,Fenfen Li +7 more
TL;DR: Wang et al. as mentioned in this paper reviewed the role of circRNAs and their role in the tumor in different dimensions, including their role as molecular sponge for miRNAs, binding with proteins, and directing selective splicing.
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