Journal Article10.1038/NRM3085
Ciliogenesis: building the cell's antenna
968
TL;DR: As cilia are important in sensing and processing developmental signals and directing the flow of fluids such as mucus, defects in ciliogenesis and length control are likely to underlie a range of cilium-related human diseases.
read more
Abstract: The cilium is a complex organelle, the assembly of which requires the coordination of motor-driven intraflagellar transport (IFT), membrane trafficking and selective import of cilium-specific proteins through a barrier at the ciliary transition zone. Recent findings provide insights into how cilia assemble and disassemble in synchrony with the cell cycle and how the balance of ciliary assembly and disassembly determines the steady-state ciliary length, with the inherent length-dependence of IFT rendering the ciliary assembly rate a decreasing function of length. As cilia are important in sensing and processing developmental signals and directing the flow of fluids such as mucus, defects in ciliogenesis and length control are likely to underlie a range of cilium-related human diseases.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Stochastic models of intracellular transport
TL;DR: A wide range of analytical methods and models of intracellular transport is presented, including Brownian ratchets, random walk models, exclusion processes, random intermittent search processes, quasi-steady-state reduction methods, and mean-field approximations for active transport.
Functions and mechanics of dynein motor proteins
TL;DR: A model for the mechanochemical cycle of dynein is emerging, in which nucleotide-driven flexing motions within the AAA+ ring of Dynein alter the affinity of its microtubule-binding stalk and reshape its mechanical element to generate movement.
The base of the cilium: roles for transition fibres and the transition zone in ciliary formation, maintenance and compartmentalization
TL;DR: Together with a recently described septin ring diffusion barrier at the ciliary base, the transition fibres and transition zone deserve attention for their varied roles in forming functional ciliary compartments.
496
Versatile protein tagging in cells with split fluorescent protein.
Daichi Kamiyama,Sayaka Sekine,Benjamin Barsi-Rhyne,Jeffrey Hu,Baohui Chen,Luke A. Gilbert,Hiroaki Ishikawa,Manuel D. Leonetti,Wallace F. Marshall,Jonathan S. Weissman,Jonathan S. Weissman,Bo Huang +11 more
TL;DR: The versatility of FP11-tag as a labelling tool as well as a multimerization-control tool for both imaging and non-imaging applications is illustrated.
Cilia Dysfunction in Lung Disease
TL;DR: Current knowledge regarding airway ciliated cells and cilia, how they function to maintain a healthy epithelium, and how disorders of cilia structure and function contribute to inherited and acquired lung disease are summarized.
References
Tetrahymena IFT122A is not essential for cilia assembly but plays a role in returning IFT proteins from the ciliary tip to the cell body
Che-Chia Tsao,Martin A. Gorovsky +1 more
TL;DR: The results indicate that Tetrahymena IFT122A is not required for anterograde transport-dependent ciliary assembly but plays a role in returning IFT proteins from the ciliary tip to the cell body.
Mechanism of transport of IFT particles in C. elegans cilia by the concerted action of kinesin-II and OSM-3 motors
Xiaoyu Pan,Guangshuo Ou,Gul Civelekoglu-Scholey,Oliver E. Blacque,Nicholas F. Endres,Li Tao,Alex Mogilner,Michel R. Leroux,Ronald D. Vale,Jonathan M. Scholey +9 more
TL;DR: Using competitive in vitro MT gliding assays, it is shown that purified kinesin-II and OSM-3 cooperate to generate movement similar to that seen along the cilium in the absence of any additional regulatory factors.
The Intraflagellar Transport Machinery of Chlamydomonas reinhardtii
TL;DR: The structure and function of the IFT machinery of Chlamydomonas reinhardtii is examined, suggesting that the particles may act as scaffolds for the binding of multiple cargoes.
Molecular architecture of axonemal microtubule doublets revealed by cryo-electron tomography
Haixin Sui,Kenneth H. Downing +1 more
TL;DR: A three-dimensional density map of intact microtubule doublets is obtained using cryo-electron tomography and image averaging, which offers insight on how tubulin protofilaments and accessory proteins attach together to form the doublets and provides a structural basis for understanding doublet function in axonemes.
Distinct IFT mechanisms contribute to the generation of ciliary structural diversity in C. elegans
TL;DR: This work examines IFT in the structurally distinct cilia of the ASH/ASI and the AWB chemosensory neurons in Caenorhabditis elegans, enabling it to be suggested that cell‐specific regulation of IFT contributes to the generation of ciliary diversity.