Journal Article10.1038/NRM3085
Ciliogenesis: building the cell's antenna
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TL;DR: As cilia are important in sensing and processing developmental signals and directing the flow of fluids such as mucus, defects in ciliogenesis and length control are likely to underlie a range of cilium-related human diseases.
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Abstract: The cilium is a complex organelle, the assembly of which requires the coordination of motor-driven intraflagellar transport (IFT), membrane trafficking and selective import of cilium-specific proteins through a barrier at the ciliary transition zone. Recent findings provide insights into how cilia assemble and disassemble in synchrony with the cell cycle and how the balance of ciliary assembly and disassembly determines the steady-state ciliary length, with the inherent length-dependence of IFT rendering the ciliary assembly rate a decreasing function of length. As cilia are important in sensing and processing developmental signals and directing the flow of fluids such as mucus, defects in ciliogenesis and length control are likely to underlie a range of cilium-related human diseases.
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Christine Blaineau,Magali Tessier,Pascal Dubessay,Lena Tasse,Lucien Crobu,Michel Pagès,Patrick Bastien +6 more
TL;DR: This is the first report of an effector protein involved in FL control through a direct action in MT dynamics, thus this finding complements the assembly-disassembly model.
Functional Genomics of the Cilium, a Sensory Organelle
Oliver E. Blacque,Elliot A. Perens,Keith A. Boroevich,Peter N. Inglis,Chunmei Li,Adam Warner,Jaswinder Khattra,Robert A. Holt,Guangshuo Ou,Allan K. Mah,Sheldon J. McKay,Peter Huang,Peter Swoboda,Steve J.M. Jones,Marco A. Marra,David L. Baillie,Donald G. Moerman,Shai Shaham,Michel R. Leroux +18 more
TL;DR: These findings help define a ciliary transcriptome and suggest that DYF-13, an evolutionarily conserved protein, is a novel core IFT component required for cilia function.
Cystin Localizes to Primary Cilia via Membrane Microdomains and a Targeting Motif
Binli Tao,Su Bu,Zhihua Yang,Brian J. Siroky,John C. Kappes,Andreas Kispert,Lisa M. Guay-Woodford +6 more
TL;DR: This work characterized the localization of cystin in the embryonic kidney and liver, in isolated renal collecting ducts, and in an inner medullary collecting duct mouse cell line and identified an AxEGG motif that is necessary to target and retain cyst in the cilium.
Defective flagellar assembly and length regulation in LF3 null mutants in Chlamydomonas.
TL;DR: Four long-flagella genes are important for flagellar length control in Chlamydomonas reinhardtii and two new null lf3 mutants are characterized whose phenotypes are different from previously identified l f3 mutants, which have unequal-length flagella that assemble more slowly than wild-type flageella.