Characterization of the temperate bacteriophage phi adh and plasmid transduction in Lactobacillus acidophilus ADH.
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TL;DR: These results demonstrated classic lytic and lysogenic cycles of replication for the temperate phage phi adh induced from L. acidophilus ADH and the first description of plasmid transduction in the genus Lactobacillus.
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Abstract: Lactobacillus acidophilus ADH is lysogenic and harbors an inducible prophage, phi adh Bacteriophage were detected in cell lysates induced by treatment with mitomycin C or UV light Electron microscopy of lysates revealed phage particles with a hexagonal head (62 nm) and a long, noncontractile, flexible tail (398 nm) ending in at last five short fibers Phage phi adh was classified within Bradley's B1 phage group and the Siphoviridae family The phi adh genome is a linear double-stranded DNA molecule of 417 kilobase pairs with cohesive ends: a physical map of the phi adh genome was constructed A prophage-cured derivative of strain ADH, designated NCK102, was isolated from cells that survived UV exposure NCK102 did not exhibit mitomycin C-induced lysis, but broth cultures lysed upon addition of phage Phage phi adh produced clear plaques on NCK102 in media containing 10 mM CaCl2 at pH values between 52 and 55 A relysogenized derivative (NCK103) of NCK102 was isolated that exhibited mitomycin C-induced lysis and superinfection immunity to phage phi adh Hybridization experiments showed that the phi adh genome was present in the ADH and NCK103 chromosomes, but absent in NCK102 These results demonstrated classic lytic and lysogenic cycles of replication for the temperate phage phi adh induced from L acidophilus ADH Phage phi adh also mediates transduction of plasmid DNA Transductants of strain ADH containing pC194, pGK12, pGB354, and pVA797 were detected at frequencies in the range of 36 x 10(-8) to 83 x 10(-10) per PFU Rearrangements or deletions were not detected in these plasmids as a consequence of transduction This is the first description of plasmid transduction in the genus Lactobacillus
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Citations
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Plasmid transduction in Streptococcus thermophilus
TL;DR: Plasmid transduction by ϕ 17 α and ϕ56α, two virulent phages of industrial yogurt starter strains are reported, which shows that several replicons could be transduced, independently of their size, with efficiencies which are high enough to allow gene transfer from a transformable intermediary strain of S. thermophilus to other hosts of the bacteriophages studied.
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Lactobacillus casei phage PL-1. Molecular properties and first transcription studies in vivo and in vitro.
TL;DR: Preliminary analysis in vitro shows an asymmetric and almost complete transcription of PL-1 DNA by the host RNA polymerase, and in vivo evidence for an early-late regulation of transcription.
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Construction, transfer and properties of a novel temperature-sensitive integrable plasmid for genomic analysis of Staphylococcus aureus
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