Journal Article10.1021/NL303163Y
Characterization of the cell-nanopillar interface by transmission electron microscopy.
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TL;DR: A detailed characterization of the cell-nanopillar interface by transmission electron microscopy finds a tight interaction between the cell membrane and the nanopillars and previous findings of excellent sealing in electrophysiology recordings using nanopillar electrodes.
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Abstract: Vertically aligned nanopillars can serve as excellent electrical, optical and mechanical platforms for biological studies. However, revealing the nature of the interface between the cell and the nanopillar is very challenging. In particular, a matter of debate is whether the cell membrane remains intact around the nanopillar. Here we present a detailed characterization of the cell-nanopillar interface by transmission electron microscopy. We examined cortical neurons growing on nanopillars with diameter 50-500 nm and heights 0.5-2 μm. We found that on nanopillars less than 300 nm in diameter, the cell membrane wraps around the entirety of the nanopillar without the nanopillar penetrating into the interior of the cell. On the other hand, the cell sits on top of arrays of larger, closely spaced nanopillars. We also observed that the membrane-surface gap of both cell bodies and neurites is smaller for nanopillars than for a flat substrate. These results support a tight interaction between the cell membrane and the nanopillars and previous findings of excellent sealing in electrophysiology recordings using nanopillar electrodes.
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References
Three-Dimensional Super-Resolution Imaging by Stochastic Optical Reconstruction Microscopy
TL;DR: 3D stochastic optical reconstruction microscopy (STORM) is demonstrated by using optical astigmatism to determine both axial and lateral positions of individual fluorophores with nanometer accuracy, allowing the 3D morphology of nanoscopic cellular structures to be resolved.
Highly efficient capture of circulating tumor cells by using nanostructured silicon substrates with integrated chaotic micromixers.
Shutao Wang,Kan Liu,Jian Liu,Jian Liu,Zeta Tak For Yu,Xiaowen Xu,Libo Zhao,Thomas H. Lee,Eun Kyung Lee,Jean Reiss,Yi-Kuen Lee,Leland W.K. Chung,Jiaoti Huang,Matthew Rettig,David Seligson,Kumaran N. Duraiswamy,Clifton K.-F. Shen,Hsian-Rong Tseng +17 more
TL;DR: In this paper, a variety of enrichment mechanisms such as immunomagnetic separation based on capture agent-labeled magnetic beads, microfluidics-based technologies that enhance cell-surface contacts, and microfilter devices that isolate CTCs based on size difference are proposed.
Vertical silicon nanowires as a universal platform for delivering biomolecules into living cells
Alex K. Shalek,Jacob T. Robinson,Ethan S. Karp,Jin Seok Lee,Dae-Ro Ahn,Myung-Han Yoon,Amy K. Sutton,Marsela Jorgolli,Rona S. Gertner,Taranjit S. Gujral,Gavin MacBeath,Eun Gyeong Yang,Hongkun Park +12 more
TL;DR: It is shown that this platform can be used to guide neuronal progenitor growth with small molecules, knock down transcript levels by delivering siRNAs, inhibit apoptosis using peptides, and introduce targeted proteins to specific organelles.
616
Vertical nanowire electrode arrays as a scalable platform for intracellular interfacing to neuronal circuits
Jacob T. Robinson,Marsela Jorgolli,Alex K. Shalek,Myung-Han Yoon,Rona S. Gertner,Hongkun Park +5 more
TL;DR: It is shown that the scalability of this platform, combined with its compatibility with silicon nanofabrication techniques, provides a clear path towards simultaneous, high-fidelity interfacing with hundreds of individual neurons.
Interfacing silicon nanowires with mammalian cells
TL;DR: This work presents the first demonstration of a direct interface of silicon nanowires with mammalian cells such as mouse embryonic stem cells and human embryonic kidney cells without any interference with each other.
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