Book Chapter10.1016/B978-0-12-814020-8.00008-1
Chapter 8 – The Zebrafish
Domenico Ribatti
- 01 Jan 2018
pp 75-88
2
TL;DR: Zebrafish will represent an important tool for chemical discovery and gene targeting in tumor angiogenesis and high-resolution in vivo imaging techniques are also becoming available in zebrafish.
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Abstract: Zebrafish (Danio rerio) represents a powerful model system to investigate tumor angiogenesis. Experimental models have been established in zebrafish adults, juveniles, and embryos. Novel genetic tools and high-resolution in vivo imaging techniques are also becoming available in zebrafish. Zebrafish angiogenesis assays are conducted by injecting a test substance (protein/peptide) into the yolk sac of the embryos, while lipophilic test substances added to the water can freely diffuse into the embryos. The transparency allows to measure angiogenesis by visual inspection. Small molecules inhibit angiogenesis already after only 24 h of development, reducing the assay time compared to other angiogenesis models. Zebrafish will represent an important tool for chemical discovery and gene targeting in tumor angiogenesis.
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Citations
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TL;DR: The human PDX1 locus is introduced into the zebrafish genome, therefore providing a humanized animal model to perform in vivo studies and elucidating the molecular mechanisms contributing to T2D susceptibility.
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References
Effective targeted gene ‘knockdown’ in zebrafish
TL;DR: It is shown here that antisense, morpholino-modified oligonucleotides (morpholinos) are effective and specific translational inhibitors in zebrafish, and conserved vertebrate processes and diseases are now amenable to a systematic, in vivo, reverse-genetic paradigm using zebra fish embryos.
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In vivo imaging of embryonic vascular development using transgenic zebrafish.
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Genes expressed in human tumor endothelium
Brad St. Croix,Carlo Rago,Carlo Rago,Victor E. Velculescu,Giovanni Traverso,Katharine E. Romans,Elizabeth A. Montgomery,Anita Lal,Gregory J. Riggins,Christoph Lengauer,Bert Vogelstein,Bert Vogelstein,Kenneth W. Kinzler +12 more
TL;DR: It is demonstrated that tumor and normal endothelium are distinct at the molecular level, a finding that may have significant implications for the development of anti-angiogenic therapies.
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Transparent Adult Zebrafish as a Tool for In Vivo Transplantation Analysis
Richard M. White,Anna Sessa,Christopher J. Burke,Teresa V. Bowman,Jocelyn LeBlanc,Craig J. Ceol,Caitlin Bourque,Michael Dovey,Michael Dovey,Wolfram Goessling,Wolfram Goessling,Caroline E. Burns,Leonard I. Zon,Leonard I. Zon +13 more
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The vascular anatomy of the developing zebrafish: an atlas of embryonic and early larval development.
TL;DR: A highly dynamic but also highly stereotypic pattern of vascular connections is found, with different sets of primitive embryonic vessels severing connections and rewiring in new configurations according to a reproducible plan.
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