Cellular localization of immunoglobulins with different allotypic specificities in rabbit lymphoid tissues
TL;DR: The cellular localization of allotypes in rabbit lymphoid tissues has been studied by immunofluorescence and double staining for two allotypes controlled by genes at different loci has shown the presence of cells containing immunoglobulins that lack one allotype.
read more
Abstract: The cellular localization of allotypes in rabbit lymphoid tissues has been studied by immunofluorescence. In heterozygous animals the double staining for two allotypes controlled by allelic genes (A1 and A2; A4 and A5; A4 and A6) has shown the existence of two populations of plasma cells, one containing one allotype and the other the alternative one. The localization in different cells of immunoglobulins marked by allelic allotypic specificities has been confirmed by microspectrography of single cells. An exception to this rule was given by the presence in the germinal centers of lymphoid follicles of apparently uniform mixtures of products of the two allelic genes.
Double staining for two allotypes controlled by genes at different loci showed, instead, the presence of many cells containing both allotypes; the number of these cells was highest in doubly homozygotes, in the other it was consistent with random association of non-allelic specificities.
In addition double staining for one allotype and gamma G globulins in the lymphoid tissues of rabbits homozygous at the a or at the b locus, has shown the presence of cells containing immunoglobulins that lack one allotype.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Host Genotype and Antibody Formation
B. Říhová-Škárová,I. Říha +1 more
- 01 Jan 1972
TL;DR: This uniformity in antibody response in inbred strains, apparent mainly after maximal antigenic stimulation, shows that variability resulting from personal history manifests itself predominantly in reactions to weak stimuli (where previous contact with cross-reacting antigens plays a decesive role).
5
Allotypes and Idiotypes
Henry G. Kunkel,Thomas Kindt +1 more
- 01 Jan 1975
TL;DR: The main objective of this review is to cover the more significant points regarding immunoglobulin (Ig) allotypes and idiotypes in different species.
5
2 BCR or NOT 2 BCR - receptor dilution: a unique mechanism for preventing the development of holes in the protective B cell repertoire.
TL;DR: This manuscript reviews the literature as it pertains to B cells and provides a summary that supports the notion that in certain situations, the over-arching rules by which the development and response of immune cells may be compromised.
5
CTCF-Mediated Genome Architecture Regulates the Dosage of Mitotically Stable Mono-allelic Expression of Autosomal Genes.
Keerthivasan Raanin Chandradoss,Bindia Chawla,Shivnarayan Dhuppar,Rakhee Nayak,Rajesh Ramachandran,Sreenivasulu Kurukuti,Aprotim Mazumder,Kuljeet Singh Sandhu +7 more
TL;DR: It is proposed that CTCF functions as a typical insulator on inactive alleles, but facilitates transcription through enhancer-linking on active allele of MAE genes, indicating widespread allele-specific regulatory roles of CTCf.
5
References
Localization of antigen in tissue cells ii. improvements in a method for the detection of antigen by means of fluorescent antibody
Albert H. Coons,Melvin H. Kaplan +1 more
TL;DR: Improvements in a method for the specific microscopic localization of antigen in tissue cells are described and two isomeric series derived from nitrofluorescein are described.
1.8K
Studies on antibody production i. a method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit
TL;DR: Small amounts of antibody were occasionally visible in cells in the lymphoid follicles of the spleen and lymph nodes, so that a minor contribution by lymphocytes to antibody synthesis cannot be excluded.
1.6K
Allotypy of rabbit serum proteins : i. immunochemical analysis leading to the individualization of seven main allotypes
TL;DR: It was concluded that at least one serum protein antigen, the specificity of which so far has been considered to be uniform throughout the animal species, can instead be present in different individuals as different forms or allotypes with somewhat different antigenic specificities.
223