Cellular localization of immunoglobulins with different allotypic specificities in rabbit lymphoid tissues
TL;DR: The cellular localization of allotypes in rabbit lymphoid tissues has been studied by immunofluorescence and double staining for two allotypes controlled by genes at different loci has shown the presence of cells containing immunoglobulins that lack one allotype.
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Abstract: The cellular localization of allotypes in rabbit lymphoid tissues has been studied by immunofluorescence. In heterozygous animals the double staining for two allotypes controlled by allelic genes (A1 and A2; A4 and A5; A4 and A6) has shown the existence of two populations of plasma cells, one containing one allotype and the other the alternative one. The localization in different cells of immunoglobulins marked by allelic allotypic specificities has been confirmed by microspectrography of single cells. An exception to this rule was given by the presence in the germinal centers of lymphoid follicles of apparently uniform mixtures of products of the two allelic genes.
Double staining for two allotypes controlled by genes at different loci showed, instead, the presence of many cells containing both allotypes; the number of these cells was highest in doubly homozygotes, in the other it was consistent with random association of non-allelic specificities.
In addition double staining for one allotype and gamma G globulins in the lymphoid tissues of rabbits homozygous at the a or at the b locus, has shown the presence of cells containing immunoglobulins that lack one allotype.
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Citations
Immunoglobulin expression in diploid and polyploid interspecies hybrids of Xenopus: evidence for allelic exclusion
Louis Du Pasquier,Ellen Hsu +1 more
TL;DR: The expression of species‐specific surface IgM was studied in various Xenopus species hybrids, suggesting that the selection pressure to make a lymphocyte synthesize only one antibody is high and that allelic exclusion already existed in the ancestors of amphibians.
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TL;DR: B cells which had been the targets for anti-M1 antibody mediated suppression are also subject to allelic exclusion.
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Ig gene expression and regulation in lg transgenic mice
Ursula Storb
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TL;DR: The importance of the analysis of the molecular and cellular mechanisms underlying Ig gene rearrangement, B cell development, feedback inhibition of heavy chain gene rearranged, and V(D)J recombinase production, and somatic hypermutation is described.
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TCRβ Feedback Signals Inhibit the Coupling of Recombinationally Accessible Vβ14 Segments with DJβ Complexes
Katherine S. Yang-Iott,Andrea C. Carpenter,Marta A. W. Rowh,Natalie C. Steinel,Brenna L. Brady,Konrad Hochedlinger,Rudolf Jaenisch,Craig H. Bassing +7 more
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TL;DR: In this paper, the authors show that in Paramecium aurelia, allelic exclusion does not depend on discrete regulatory genes dispersed throughout the genome and does not seem to be influenced by cytoplasmic factors.
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