Cellular localization of immunoglobulins with different allotypic specificities in rabbit lymphoid tissues
TL;DR: The cellular localization of allotypes in rabbit lymphoid tissues has been studied by immunofluorescence and double staining for two allotypes controlled by genes at different loci has shown the presence of cells containing immunoglobulins that lack one allotype.
read more
Abstract: The cellular localization of allotypes in rabbit lymphoid tissues has been studied by immunofluorescence. In heterozygous animals the double staining for two allotypes controlled by allelic genes (A1 and A2; A4 and A5; A4 and A6) has shown the existence of two populations of plasma cells, one containing one allotype and the other the alternative one. The localization in different cells of immunoglobulins marked by allelic allotypic specificities has been confirmed by microspectrography of single cells. An exception to this rule was given by the presence in the germinal centers of lymphoid follicles of apparently uniform mixtures of products of the two allelic genes.
Double staining for two allotypes controlled by genes at different loci showed, instead, the presence of many cells containing both allotypes; the number of these cells was highest in doubly homozygotes, in the other it was consistent with random association of non-allelic specificities.
In addition double staining for one allotype and gamma G globulins in the lymphoid tissues of rabbits homozygous at the a or at the b locus, has shown the presence of cells containing immunoglobulins that lack one allotype.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Molecular requirements for the mu-induced light chain gene rearrangement in pre-B cells.
TL;DR: It is proposed that the protein complex composed of mu and the 33 kd/36 kd protein heterodimer is responsible for the activation of the L chain gene locus and its rearrangement.
66
Pre-B and B cells in rabbits. Ontogeny and allelic exclusion of kappa light chain genes.
TL;DR: Pre-B cells in developing rabbits were identified by immunofluorescence as cells containing small amounts of cytoplasmic IgM but lacking surface immunoglobulin (sIg).
65
Immunoglobulin allotypic determinants on rabbit lymphocytes.
TL;DR: Direct evidence is obtained for the presence of immunoglobulin determinants on the surface of rabbit lymphocytes by autoradiography using radio-labelled anti-allotype sera.
63
Immunoglobulin genes in transgenic mice
TL;DR: This work has demonstrated that a mouse can be genetically engineered to produce a desired immune product and the mechanisms which control the somatic assembly and expression of immunoglobulin genes are examined.
63
Monoallelic expression and asynchronous replication of p120 catenin in mouse and human cells.
TL;DR: It is demonstrated that asynchronous DNA replication can be used as a marker for the identification of novel genes with monoallelic expression and p120 catenin, a gene involved in cell adhesion, is identified as belonging to this class.
63
References
Localization of antigen in tissue cells ii. improvements in a method for the detection of antigen by means of fluorescent antibody
Albert H. Coons,Melvin H. Kaplan +1 more
TL;DR: Improvements in a method for the specific microscopic localization of antigen in tissue cells are described and two isomeric series derived from nitrofluorescein are described.
1.8K
Studies on antibody production i. a method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit
TL;DR: Small amounts of antibody were occasionally visible in cells in the lymphoid follicles of the spleen and lymph nodes, so that a minor contribution by lymphocytes to antibody synthesis cannot be excluded.
1.6K
Allotypy of rabbit serum proteins : i. immunochemical analysis leading to the individualization of seven main allotypes
TL;DR: It was concluded that at least one serum protein antigen, the specificity of which so far has been considered to be uniform throughout the animal species, can instead be present in different individuals as different forms or allotypes with somewhat different antigenic specificities.
223