Journal Article10.1016/0022-1759(92)90316-L
Cellular enzyme-linked immunocircle assay. A rapid assay of hybridomas produced against cell surface antigens
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TL;DR: A novel method has been developed for the initial screening of hybridomas produced against cell surface antigens using existing rat monoclonal antibodies and new rat-mouse hybridoma cell lines against mouse thymocytes and bone marrow cells.
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About: This article is published in Journal of Immunological Methods. The article was published on 30 Aug 1992. The article focuses on the topics: Monoclonal antibody & Antigen.
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Citations
Cigarette Smoke-Induced Pulmonary Inflammation Becomes Systemic by Circulating Extracellular Vesicles Containing Wnt5a and Inflammatory Cytokines.
D. Feller,József Kun,Istvan Ruzsics,Judit Rapp,Veronika Sárosi,Krisztian Kvell,Zsuzsanna Helyes,Judit E. Pongracz +7 more
TL;DR: It is shown that non-canonical and pro-inflammatory Wnt5a is up-regulated by cigarette smoking with parallel up-regulation of pro- inflammatory cytokines in both mouse and human model systems, demonstrating a potential mechanism for the systemic nature of this crippling disease.
A cell surface ELISA for the screening of monoclonal antibodies to antigens on viable cells in suspension.
TL;DR: The cell ELISA was found to be as sensitive and specific as the fluorescence assay when analysing different cell lines with selected antibodies and could be increased using higher cell numbers and conjugate concentration.
28
Detection of phenotypic heterogeneity within the murine splenic vasculature using rat monoclonal antibodies IBL-7/1 and IBL-7/22.
TL;DR: These two new rat monoclonal antibodies may be valuable tools for the phenotypic analysis of splenic endothelium, and can be used for the identification of various endothelial cell subpopulations of the mouse spleen.
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Overexpression of Bovine FcRn in Mice Enhances T-Dependent Immune Responses by Amplifying T Helper Cell Frequency and Germinal Center Enlargement in the Spleen.
Zita Schneider,Péter K. Jani,Bence Szikora,Attila Gergely Végh,Dorottya Kövesdi,Attila Iliás,Judit Cervenak,Péter Balogh,István Kurucz,Imre Kacskovics +9 more
TL;DR: The in vivo impact of the bFcRn overexpression in the spleen on the course of antibody production was demonstrated and it was demonstrated that these Tg mice developed efficient immune response against very low dose of antigen, reflecting another important practical benefit of these TG mice.
Novel method for in vitro depletion of T cells by monoclonal antibody-targeted photosensitization
Timea Berki,Péter Németh +1 more
TL;DR: The selective killing of T lymphocytes (bearing the Thy-1 antigen) in a mixed cell population was demonstrated after a treatment with the phototoxic conjugate and light irradiation.
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References
Continuous cultures of fused cells secreting antibody of predefined specificity
G Köhler,C. Milstein +1 more
TL;DR: The derivation of a number of tissue culture cell lines which secrete anti-sheep red blood cell (SRBC) antibodies is described here, made by fusion of a mouse myeloma and mouse spleen cells from an immunised donor.
20.3K
Enzyme immunoassay ELISA and EMIT.
TL;DR: This chapter discusses the enzyme immunoassay ELISA and EMIT, where ELISA requires very little knowledge of enzyme technology, whereas enzymology is the key to success in EMIT.
1K
•Book
Immunochemistry in practice
Alan P. Johnstone,Robin Thorpe +1 more
- 01 Jan 1982
TL;DR: Immunochemistry in practice, Immunochemical in practice , مرکز فناوری اطلاعات و اصاع رسانی, کδاوρزی
654
Cytochemical demonstration of peroxidase activity with 3-amino-9-ethylcarbazole.
TL;DR: Slides stained with dilute Lugol’s iooline to cont rod the localization of pimosphorylase activity were again washed in running water to remove iodine and these treatments remove glucose-i-phosphate muelsorbed by time tissue section.
568
Enzyme-mediated immunoassay
That T. Ngo,Howard M. Lenhoff +1 more
- 01 Jan 1985
TL;DR: Separation-Free Enzyme Fluorescence Immunoassay by Continuous Flow Injection Analysis and Development of Immunochemical Enzyme Assays for Cardiac Isoenzymes.
90