Cellular Cholesterol Efflux Mediated by Cyclodextrins DEMONSTRATION OF KINETIC POOLS AND MECHANISM OF EFFLUX
Patricia G. Yancey,Wendi V. Rodrigueza,E. P. C. Kilsdonk,Genevieve W. Stoudt,William J. Johnson,Michael C. Phillips,George H. Rothblat +6 more
652
TL;DR: It seems that cyclodextrin molecules are substantially more efficient than phospholipid acceptors, because cholesterol molecules desorbing from a membrane surface can diffuse directly into the hydrophobic core of a cyclodeXTrin molecule without having to desorb completely into the aqueous phase before being sequestered by the acceptor.
read more
About: This article is published in Journal of Biological Chemistry. The article was published on 05 Jul 1996. and is currently open access. The article focuses on the topics: Cyclodextrin & Efflux.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
The Inseparable Relationship Between Cholesterol and Hedgehog Signaling.
Christian Siebold,Rajat Rohatgi +1 more
TL;DR: A remarkable feature of the Hedgehog pathway is the repeated use of cholesterol in steps spanning ligand biogenesis, secretion, dispersal, and reception on target cells as discussed by the authors , which supports the view that the pathway likely evolved by coopting ancient systems to regulate the abundance or organization of sterol-like lipids in membranes.
Cholesterol Reporter Molecules
Gerald Gimpl,Katja Gehrig-Burger +1 more
TL;DR: Different approaches to detect cholesterol in vitro and in vivo are described and the features of the different cholesterol reporter molecules are critically discussed with a special focus on recent imaging approaches.
Cholesterol Removal by Methyl-β-Cyclodextrin Inhibits Poliovirus Entry
Pranav Danthi,Marie Chow +1 more
TL;DR: It is shown that methyl-β-cyclodextrin (MβCD), which disrupts DIMs by depleting cells of cholesterol, inhibits virus infection and that this inhibition was partially reversed by partially restoring cholesterol levels in cells, suggesting that MβCD inhibition of virus infection was mediated by removal of cellular cholesterol.
Cyclodextrins but not compactin inhibit the lateral diffusion of membrane proteins independent of cholesterol.
TL;DR: It is suggested that the widely used treatment with CD to reduce cholesterol has additional, cholesterol‐independent effects on membrane protein mobility, which do not necessarily distinguish between raft and non‐raft proteins.
Distinct modes of molecular regulation of CCL3 induced calcium flux in monocytic cells.
TL;DR: It is shown that CCR5 signalling is dependent on thapsigargin-sensitive Ca2+ stores and on activation of ryanodine receptors as well as InsP3 receptors or store-operated channels and the loss of response in lovastatin treated cells can be rescued by MCD addition, which shows that the cholesterol content in the membrane is only one factor in determining the amount of receptor response.
References
•Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
318.1K
A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples
TL;DR: The original Lowry method of protein determination has been modified by the addition of sodium dodecyl sulfate in the alkali reagent and an increase in the amount of copper tartrate reagent to be used with membrane and lipoprotein preparations without prior solubilization or lipid extraction.
6.7K
The plasma lecithin:cholesterol acyltransferase reaction
TL;DR: Several indications exist that the acyltransferase reaction is the major source of plasma esterified cholesterol in man, and one possibility is that it plays a role in the transport of cholesterol from peripheral tissues to the liver.
2.5K
Molecular physiology of reverse cholesterol transport.
TL;DR: The removal of cholesterol from cells, like its delivery, appears to be specific and well regulated, and RCT can now be understood in molecular terms.
1.5K
Practical methods for plasma lipoprotein analysis.
TL;DR: In this paper, the authors discuss the practical methods for plasma lipoprotein analysis and discuss the changes in the concentration and sometimes in the nature of the lipoproteins occur in a variety of diseases.
1.3K