Cellular analysis using label-free parallel array microscopy with Fourier ptychography
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TL;DR: Compared to manually counted fluorescence ground-truth, cell counting results after automated segmentation showed improved accuracy with QPI over DPC, and FPM showed improvement in the ability to resolve fine details and thin cells, despite limitations of the FPM system incurred by imaging artifacts.
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Abstract: : Quantitative phase imaging (QPI) is an ideal method to non-invasively monitor cell populations and provide label-free imaging and analysis. QPI offers enhanced sample characteri-zation and cell counting compared to conventional label-free techniques. We demonstrate this in the current study through a comparison of cell counting data from digital phase contrast (DPC) imaging and from QPI using a system based on Fourier ptychographic microscopy (FPM). Our FPM system offers multi-well, parallel imaging and a QPI-specific cell segmentation method to establish automated and reliable cell counting. Three cell types were studied and FPM showed improvement in the ability to resolve fine details and thin cells, despite limitations of the FPM system incurred by imaging artifacts. Relative to manually counted fluorescence ground-truth, cell counting results after automated segmentation showed improved accuracy with QPI over DPC.
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Citations
Beyond conventional microscopy: Observing kidney tissues by means of fourier ptychography
Maria Rita Valentino,Vittorio Bianco,Lisa Miccio,Pasquale Memmolo,V. Brancato,Paolo Libretti,Marcello Gambacorta,Marco Salvatore,Pietro Ferraro +8 more
TL;DR: In this article , Fourier Ptychography (FP) has been used to obtain high-resolution images of kidney tissues and in vitro cells with a wide field of view.
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