Journal Article10.1111/J.1349-7006.2010.01735.X
CD10-bearing fibroblast inhibits matrigel invasive potency of interleukin-1α-producing squamous cell carcinoma by diminishing substance P levels in the tumor microenvironment.
Lining Xie,Yoichi Moroi,Gaku Tsuji,Min Liu,Sayaka Hayashida,Masakazu Takahara,Shuji Fukagawa,Satoshi Takeuchi,Baoen Shan,Takeshi Nakahara,Hiroshi Uchi,Takehiko Yokomizo,Masutaka Furue +12 more
TL;DR: The CD10‐bearing Fb generated by SCC‐derived IL‐1 inhibited the invasive capacity of SCC by diminishing the microenvironmental concentration of substance P.
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Abstract: CD10 is a neutral endopeptidase, which cleaves various peptide substrates including substance P. CD10 expression has been detected in peritumoral fibroblasts (Fb) within the invasive area of various cancers such as squamous cell carcinoma (SCC). However, the biological significance of CD10-bearing Fb remains largely unknown. We examined dynamic interactions of Fb with tumorigenic A431 SCC cells or non-tumorigenic HaCaT squamous cells. The SCC and HaCaT cells did not synthesize CD10, while Fb constitutively expressed CD10. When co-cultured, SCC markedly upregulated fibroblastic CD10 expression compared with HaCaT, which was mainly attributable to SCC-derived interleukin-1α (IL-1α). Both SCC and Fb autonomously secreted substance P, which eventually enhanced the invasive capacity of SCC in a matrigel invasion assay by upregulating matrix metalloproteinase (MMP)-1 and MMP-2, but not MMP-9. Transfection of siRNA for CD10 successfully knocked down the CD10 expression in Fb (CD10ND-Fb). In the presence of CD10ND-Fb, substance P levels in supernatants as well as MMP production and the invasive potency of SCC were significantly augmented compared with control scramble RNA-transfected Fb. We also transfected CD10 vector to Fb and found that the matrigel invasive ability of SCC cells was downregulated co-cultured with CD10 vector-transfected Fb rather than empty vector-transfected Fb. In conclusion, the CD10-bearing Fb generated by SCC-derived IL-1 inhibited the invasive capacity of SCC by diminishing the microenvironmental concentration of substance P. (Cancer Sci 2010; 101: 2570–2578)
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Influence of sensory neuropeptides on human cutaneous wound healing process
Jérémy Chéret,Nicolas Lebonvallet,Virginie Buhé,Jean-Luc Carré,Laurent Misery,C. Le Gall-Ianotto +5 more
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The Neurokinin-1 Receptor Antagonist Aprepitant: An Intelligent Bullet against Cancer?
Miguel Muñoz,Rafael Coveñas +1 more
TL;DR: The data support the reprofiling of aprepitant for a new therapeutic use as an antitumor agent and the use of this drug is suggested in cancer patients.
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Substance P accelerates the progression of human esophageal squamous cell carcinoma via MMP-2, MMP-9, VEGF-A, and VEGFR1 overexpression.
TL;DR: To assess the effects of the FDA-approved aprepitant drug, a commercially available NK-1R antagonist, on the viability of KYSE-30 ESCC cells, resazurin assay was performed and the influence of SP/NK- 1R system on the migration potential of these cells was examined using scratch assay.
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CD10 in the developing human kidney: immunoreactivity and possible role in renal embryogenesis.
Gavino Faa,Clara Gerosa,D Fanni,Sonia Nemolato,Viviana Marinelli,Annalisa Locci,Giancarlo Senes,Valerio Mais,P Van Eyken,Nicoletta Iacovidou,Guido Monga,V. Fanos +11 more
TL;DR: The data show a strong expression of CD10 in all stage of human kidney development, characterized by dynamic changes and support the hypothesis that CD10 plays a relevant role in renal embryogenesis.
27
Regulation of Carcinogenesis by Sensory Neurons and Neuromediators
TL;DR: Local and systemic effects of sensory nerve activity as well as Schwann cells in carcinogenesis and metastasis are mainly focused on, with several studies having reported that inhibition of sensory neuronal activity reduces the development of solid tumors.
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