cd1 Mutation in Drosophila Affects Phenoxazinone Synthase Catalytic Site and Impairs Long-Term Memory
Aleksandr V. Zhuravlev,P. N. Ivanova,Ksenia A. Makaveeva,Gennadii A. Zakharov,Ekaterina A. Nikitina,E. V. Savvateeva-Popova +5 more
TL;DR: It is shown that the aberrant PHS sequence in cd1 provokes drastic LTM impairments and DAN alterations, in accordance with the concept “from the gene to behavior” proclaimed by S. Benzer.
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Abstract: Being involved in development of Huntington’s, Parkinson’s and Alzheimer’s diseases, kynurenine pathway (KP) of tryptophan metabolism plays a significant role in modulation of neuropathology. Accumulation of a prooxidant 3-hydroxykynurenine (3-HOK) leads to oxidative stress and neuronal cell apoptosis. Drosophila mutant cardinal (cd1) with 3-HOK excess shows age-dependent neurodegeneration and short-term memory impairments, thereby presenting a model for senile dementia. Although cd gene for phenoxazinone synthase (PHS) catalyzing 3-HOK dimerization has been presumed to harbor the cd1 mutation, its molecular nature remained obscure. Using next generation sequencing, we have shown that the cd gene in cd1 carries a long deletion leading to PHS active site destruction. Contrary to the wild type Canton-S (CS), cd1 males showed defective long-term memory (LTM) in conditioned courtship suppression paradigm (CCSP) at days 5–29 after eclosion. The number of dopaminergic neurons (DAN) regulating fly locomotor activity showed an age-dependent tendency to decrease in cd1 relative to CS. Thus, in accordance with the concept “from the gene to behavior” proclaimed by S. Benzer, we have shown that the aberrant PHS sequence in cd1 provokes drastic LTM impairments and DAN alterations.
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Extension of life span by down-regulation of enzymes catalyzing tryptophan conversion into kynurenine: Possible implications for mechanisms of aging
TL;DR: In this article , the authors focused on the possible role of the initial step of tryptophan catabolism, kynurenine (Kyn) formation from Trp, in aging mechanism(s).
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Courtship suppression in Drosophila melanogaster: The role of mating failure
Anna A Goncharova,Natalia G. Besedina,J. Bragina,L. V. Danilenkova,E. A. Kamysheva,Sergey A Fedotov +5 more
TL;DR: It is shown that the previously described mechanisms of courtship suppression, as a result of the association of the courtship object with the repellent, as well as due to increased sensitivity to the anti-aphrodisiac cVA after mating failure, are not confirmed when immature mobile females are used.
References
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