Journal Article10.1038/NBT950
Capturing and profiling adult hair follicle stem cells
Rebecca J. Morris,Yaping Liu,Lee Marles,Zaixin Yang,Carol S. Trempus,Shulan Li,Jamie S. Lin,Janet A. Sawicki,George Cotsarelis +8 more
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TL;DR: It is shown that bulge cells in adult mice generate all epithelial cell types within the intact follicle and hair during normal hair follicle cycling and provide potential targets for the treatment of hair loss and other disorders of skin and hair.
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Abstract: The hair follicle bulge possesses putative epithelial stem cells. Characterization of these cells has been hampered by the inability to target bulge cells genetically. Here, we use a Keratin1-15 (Krt1-15, also known as K15) promoter to target mouse bulge cells with an inducible Cre recombinase construct or with the gene encoding enhanced green fluorescent protein (EGFP), which allow for lineage analysis and for isolation of the cells. We show that bulge cells in adult mice generate all epithelial cell types within the intact follicle and hair during normal hair follicle cycling. After isolation, adult Krt1-15-EGFP-positive cells reconstituted all components of the cutaneous epithelium and had a higher proliferative potential than Krt1-15-EGFP-negative cells. Genetic profiling of hair follicle stem cells revealed several known and unknown receptors and signaling pathways important for maintaining the stem cell phenotype. Ultimately, these findings provide potential targets for the treatment of hair loss and other disorders of skin and hair.
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Citations
Identification of stem cells in small intestine and colon by marker gene Lgr5
Nick Barker,Johan H. van Es,Jeroen Kuipers,Pekka Kujala,Maaike van den Born,Miranda Cozijnsen,Andrea Haegebarth,Jeroen Korving,Harry Begthel,Peter J. Peters,Hans Clevers +10 more
TL;DR: The expression pattern of Lgr5 suggests that it marks stem cells in multiple adult tissues and cancers, suggesting that it represents the stem cell of the small intestine and colon.
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TL;DR: Although bronchiolar cells and alveolar cells are proposed to be the precursor cells of adenocarcinoma, this work points to bronchioalveolar stem cells as the putative cells of origin for this subtype of lung cancer.
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References
Label-retaining cells reside in the bulge area of pilosebaceous unit: Implications for follicular stem cells, hair cycle, and skin carcinogenesis
TL;DR: It is suggested that follicular stem cells reside in the bulge region, instead of the lower bulb, which provides insights into hair cycle control and the possible involvement of hair follicle stem cells in skin carcinogenesis.
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Defining the epithelial stem cell niche in skin.
Tudorita Tumbar,Géraldine Guasch,Valentina Greco,Cédric Blanpain,William E. Lowry,Michael Rendl,Elaine Fuchs +6 more
TL;DR: It is found that these cells rarely divide within their niche but change properties abruptly when stimulated to exit, and their transcriptional profile is determined, which, when compared to progeny and other SCs, defines the niche.
"Stemness": Transcriptional Profiling of Embryonic and Adult Stem Cells
TL;DR: The transcriptional profiles of mouse embryonic, neural, and hematopoietic stem cells were compared to define a genetic program for stem cells and provide a foundation for a more detailed understanding of stem cell biology.
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A Stem Cell Molecular Signature
Natalia Ivanova,John T. Dimos,Christoph Schaniel,Jason A. Hackney,Kateri A. Moore,Ihor R. Lemischka +5 more
TL;DR: This work determined global gene expression profiles for mouse and human hematopoietic stem cells and other stages of the hematopolietic hierarchy, which define key conserved regulatory pathways in this developmental system.
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Existence of slow-cycling limbal epithelial basal cells that can be preferentially stimulated to proliferate: implications on epithelial stem cells.
TL;DR: Using 3H-thymidine labeling, a subpopulation of corneal epithelial basal cells are identified in the peripheral cornea in a region called limbus that are normally slow cycling, but can be stimulated to proliferate in response to wounding and to a tumor promotor, TPA.
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