Journal Article10.1016/0092-8674(76)90128-8
Capping of eucaryotic mRNAs
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About: This article is published in Cell. The article was published on 01 Dec 1976.
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Citations
A simplified method to produce mRNAs and functional proteins from synthetic double-stranded DNA templates.
TL;DR: This work constructed five unique synthetic DNA templates, produced mRNAs and demonstrated biologic activity of their translated proteins and proposed that this method offers a cost- and time-saving alternative to plasmid-based cloning.
10
In vitro capping in Trypanosoma cruzi identifies and shows specificity for the spliced leader RNA and U-RNAs
TL;DR: The apparent specificity of this in vitro capping activity closely reflects the in vivo requirements; i.e., only the SL- and U-RNAs need to be capped since mature mRNAs are capped via trans-splicing.
Free cytoplasmic messenger ribonucleoprotein complexes from rabbit reticulocytes.
TL;DR: It was concluded that free cytoplasmic mR NP-particles from rabbit reticulocytes can be classified as “old” mRNP in a post-translational phase and it was suggested that free Cytoplasmaic mRNA has a 5′ terminal “cap”.
10
High resolution biosensor to test the capping level and integrity of mRNAs.
TL;DR: A biosensor designed to detect the presence of cap structures on mRNAs that is also sensitive to mRNA degradation, so uncapped or degraded m RNAs can be detected in a single step.
Quantifying the RNA cap epitranscriptome reveals novel caps in cellular and viral RNA
Jin Wang,Jin Wang,Bing Liang Alvin Chew,Bing Liang Alvin Chew,Yong Lai,Hongping Dong,Luang Xu,Seetharamsingh Balamkundu,Seetharamsingh Balamkundu,Weiling Maggie Cai,Weiling Maggie Cai,Liang Cui,Chuan-Fa Liu,Xin-Yuan Fu,Zhenguo Lin,Pei Yong Shi,Timothy K. Lu,Timothy K. Lu,Dahai Luo,Samie R. Jaffrey,Peter C. Dedon,Peter C. Dedon +21 more
TL;DR: CapQuant accurately captured the preference for purine nucleotides at eukaryotic transcription start sites and the correlation between metabolite levels and metabolite caps, and the mystery around cap m1A/m1Am analysis remains unresolved.
10
References
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TL;DR: Unmethylated reovirus and VSV mRNAs are specifically methylated to form 5′-terminal structures of the type, m7G ( 5′)ppp (5′) N by protein synthesizing extracts prepared from wheat germ and mouse L cells.
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