Calcium-sensing Receptor Attenuates AVP-induced Aquaporin-2 Expression via a Calmodulin-dependent Mechanism
Mauro Bustamante,Udo Hasler,Valérie Leroy,Sophie de Seigneux,Mitko Dimitrov,David Mordasini,Martine Rousselot,Pierre-Yves Martin,Eric Féraille +8 more
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TL;DR: High concentrations of extracellular calcium attenuate AVP-induced aquaporin-2 expression by activating the CaSR and reducing coupling efficiency between V(2) receptor and adenylate cyclase via a calmodulin-dependent mechanism in cultured cortical collecting duct cells.
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Abstract: Recent evidence suggests that arginine vasopressin (AVP)-dependent aquaporin-2 expression is modulated by the extracellular calcium-sensing receptor (CaSR) in principal cells of the collecting duct, but the signaling pathways mediating this effect are unknown. Using a mouse cortical collecting duct cell line (mpkCCD(cl4)), we found that increasing the concentration of apical extracellular calcium or treating with the CaSR agonists neomycin or Gd(3+) attenuated AVP-dependent accumulation of aquaporin-2 mRNA and protein; CaSR gene-silencing prevented this effect. Calcium reduced the AVP-induced accumulation of cAMP, but this did not occur by increased degradation of cAMP by phosphodiesterases or by direct inhibition of adenylate cyclase. Notably, the effect of extracellular calcium on AVP-dependent aquaporin-2 expression was prevented by inhibition of calmodulin. In summary, our results show that high concentrations of extracellular calcium attenuate AVP-induced aquaporin-2 expression by activating the CaSR and reducing coupling efficiency between V(2) receptor and adenylate cyclase via a calmodulin-dependent mechanism in cultured cortical collecting duct cells.
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The Calcium-Sensing Receptor: A Molecular Perspective
TL;DR: This review will provide a comprehensive exploration of the different aspects of the receptor, including its structure, stimuli, signalling, interacting protein partners, and tissue expression patterns, and will relate their impact on the functionality of the CaR from a molecular perspective.
239
Renal aquaporins and water balance disorders.
TL;DR: This review summarizes the current knowledge of the underlying mechanisms of various water balance disorders and their treatment strategies and opens up several possible new treatment strategies.
161
Localization and function of the renal calcium-sensing receptor
TL;DR: The kidney expresses a CaSR that might directly contribute to the regulation of many aspects of renal function in a PTH-independent manner, and the potential impact of pharmacological modulation of the CaSR on renal function is discussed.
The epithelial sodium/proton exchanger, NHE3, is necessary for renal and intestinal calcium (re)absorption
Wanling Pan,Jelena Borovac,Zachary Spicer,Joost G. J. Hoenderop,René J. Bindels,Gary E. Shull,Michael R. Doschak,Emmanuelle Cordat,R. Todd Alexander +8 more
TL;DR: Significant alterations of Ca(2+) homeostasis in NHE3(-/-) mice are demonstrated and a molecular link between Na(+) and Ca( 2+) (re)absorption is provided.
105
Identification and Molecular Mechanisms of the Rapid Tonicity-induced Relocalization of the Aquaporin 4 Channel
Philip Kitchen,Rebecca E. Day,Luke H.J. Taylor,Mootaz M. Salman,Roslyn M. Bill,Matthew T. Conner,Alex C. Conner +6 more
TL;DR: It is shown that AQP4 cell surface expression can be rapidly and reversibly regulated in response to changes of tonicity in primary cortical rat astrocytes and in transfected HEK293 cells.
103
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Long Term Regulation of Aquaporin-2 Expression in Vasopressin-responsive Renal Collecting Duct Principal Cells
Udo Hasler,David Mordasini,Marcelle Bens,Matthieu Bianchi,Françoise Cluzeaud,Martine Rousselot,Alain Vandewalle,Eric Féraille,Pierre-Yves Martin +8 more
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