Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology.
Nadanai Laohakunakorn,Laura Grasemann,Barbora Lavickova,Grégoire Michielin,Amir Shahein,Zoe Swank,Sebastian J. Maerkl +6 more
TL;DR: The history and recent developments in engineering recombinant and crude extract systems, as well as breakthroughs in enabling technologies, that have facilitated increased throughput, compartmentalization, and spatial control of cell-free protein synthesis reactions are discussed.
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Abstract: Cell-free systems offer a promising approach to engineer biology since their open nature allows for well-controlled and characterized reaction conditions. In this review, we discuss the history and recent developments in engineering recombinant and crude extract systems, as well as breakthroughs in enabling technologies, that have facilitated increased throughput, compartmentalization, and spatial control of cell-free protein synthesis reactions. Combined with a deeper understanding of the cell-free systems themselves, these advances improve our ability to address a range of scientific questions. By mastering control of the cell-free platform, we will be in a position to construct increasingly complex biomolecular systems, and approach natural biological complexity in a bottom-up manner.
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Citations
Organizational change of synthetic biology research: Emerging initiatives advancing a bottom-up approach
Renan Gonçalves Leonel da Silva,Jakob Schweizer,Kalina Kamenova,Larry Au,Alessandro Blasimme,Effy Vayena +5 more
TL;DR: The study found that publications of specialized articles on “ bottom-up synthetic biology ” have increased, both in absolute numbers and normalized to total number of publications, and shows how that might be enabled by novel mechanisms of organization that reposition material, intellectual and political resources in synthetic biology.
Effective Use of Linear DNA in Cell-Free Expression Systems.
TL;DR: In this paper, a review describes approaches to LET stabilization used in CFEs, summarizes the advancements that have come from using LETs with these methods, and identifies future applications and development goals that are likely to be impactful to the field.
Cell-Free Production Systems in Droplet Microfluidics.
TL;DR: The use of cell-free macromolecule production toolbox in droplet microfluidic devices has gained significant interest during the last decade as discussed by the authors , with a special emphasis on new on-chip technologies for the amplification, transcription, expression, screening, and directed evolution of biomolecules.
High-throughput screening of cell-free riboswitches by fluorescence-activated droplet sorting
TL;DR: A high-throughput screening method is reported to engineer cell-free riboswitches that respond to small molecules and a histamine riboswitch is used to demonstrate chemical communication between cell-sized droplets.
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