Boosting Synergistic Effects of Short Antimicrobial Peptides With Conventional Antibiotics Against Resistant Bacteria.
TL;DR: In this paper, a checkerboard method was used to evaluate synergistic effects of the parent peptide and the Nal-tagged peptides, and the synergistic mechanism of action between the peptides and vancomycin was analyzed.
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Abstract: The global spread of antibiotic-resistant infections has meant that there is an urgent need to develop new antimicrobial alternatives. In this study, we developed a strategy to boost and/or synergize the activity of conventional antibiotics by combination with antimicrobial peptides tagged with the bulky non-natural amino acid β-naphthylalanine (Nal) to their N- or C-terminus. A checkerboard method was used to evaluate synergistic effects of the parent peptide and the Nal-tagged peptides. Moreover, boron-dipyrro-methene labeled vancomycin was used to characterize the synergistic mechanism of action between the peptides and vancomycin on the bacterial strains. These Nal-tagged antimicrobial peptides also reduced the antibiotic-induced release of lipopolysaccharide from Gram-negative bacteria by more than 99.95%. Our results demonstrate that Nal-tagged peptides could help in developing antimicrobial peptides that not only have enhanced antibacterial activities but also increase the synergistic effects with conventional antibiotics against antibiotic-resistant bacteria.
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Citations
Antimicrobial peptides, conventional antibiotics, and their synergistic utility for the treatment of drug‐resistant infections
TL;DR: The progress in synergistic studies on AMPs and conventional antibiotics and an overview of the current understanding of the functional scope of AMPs, ongoing clinical trials, and challenges in the development processes are presented.
144
Flourishing Antibacterial Strategies for Osteomyelitis Therapy
TL;DR: The main targets of antibacterial agents for treating osteomyelitis, including bacterial cell wall, cell membrane, intracellular macromolecules, and bacterial energy metabolism, were introduced in this paper .
52
Antimicrobial peptides: Sustainable application informed by evolutionary constraints.
TL;DR: In this paper , the benefits, challenges, and opportunities of utilizing antimicrobial peptides against disease-causing bacteria are discussed, and highlights the use of AMP cocktails or nanomaterials to proactively address potential AMP resistance crises in the future.
22
Scutellarin potentiates vancomycin against lethal pneumonia caused by methicillin-resistant Staphylococcus aureus through dual inhibition of sortase A and caseinolytic peptidase P.
Xingye Wang,Lin Wei,Li Wang,Xiaoyu Chen,Xiangri Kong,Y. Luan,Jiyu Guan,Xuerui Guo,Yan Shi,Tiedong Wang,Bingmei Wang,Wu Song,Yicheng Zhao +12 more
TL;DR: Wang et al. as mentioned in this paper found scutellarin, a natural flavonoid, markedly inhibited SrtA and caseinolytic peptidase P activities of MRSA strain USA300 with an IC50 of 53.64 μg/mL and 107.00μm/mL, respectively.
21
Hibifolin, a Natural Sortase A Inhibitor, Attenuates the Pathogenicity of Staphylococcus aureus and Enhances the Antibacterial Activity of Cefotaxime
Wu Song,Li Wang,Yinghua Zhao,Gongga Lanzi,Xingye Wang,Chi Zhang,Jiyu Guan,Wei Wang,Xuerui Guo,Ying Meng,Bingmei Wang,Yicheng Zhao +11 more
TL;DR: It is identified that hibifolin attenuated the pathogenicity of S. aureus by directly targeting SrtA, which may be utilized in the future as adjuvant therapy for S.aureus infections.
21
References
Synergy, antagonism, and what the chequerboard puts between them
TL;DR: This document is intended to be used for educational purposes only and should not be relied on as a guide for making decisions about major decisions about copyrighted material.
Silver Enhances Antibiotic Activity Against Gram-Negative Bacteria
Jose Ruben Morones-Ramirez,Jose Ruben Morones-Ramirez,Jonathan A. Winkler,Jonathan A. Winkler,Catherine S. Spina,Catherine S. Spina,James J. Collins +6 more
TL;DR: This work shows that silver can be used to enhance the action of existing antibiotics against Gram-negative bacteria, thus strengthening the antibiotic arsenal for fighting bacterial infections.
733
Ultrashort antibacterial and antifungal lipopeptides
TL;DR: The simple composition of these lipopeptides and their diverse specificities should make them economically available, innate immunity-mimicking antimicrobial and antifungal compounds for various applications.
463
Antibiotic-resistant bacteria show widespread collateral sensitivity to antimicrobial peptides
Viktória Lázár,Viktória Lázár,Ana Martins,Réka Spohn,Lejla Daruka,Gábor Grézal,Gergely Fekete,Mónika Számel,Pramod Kumar Jangir,Bálint Kintses,Bálint Csörgő,Ákos Nyerges,Ádám Györkei,András Kincses,András Dér,Fruzsina R. Walter,Mária A. Deli,Edit Urbán,Zsófia Hegedűs,Gábor Olajos,Orsolya Méhi,Balázs Bálint,István Nagy,Tamás A. Martinek,Balázs Papp,Csaba Pál +25 more
TL;DR: The identification of antimicrobial peptide–antibiotic combinations that enhance antibiotic activity against multidrug-resistant bacteria and slow down de novo evolution of resistance are identified and guidelines for the development of efficient peptide-based therapies of antibiotic-resistant infections are provided.
An overview of antimicrobial peptides and the latest advances in their development
TL;DR: An overview of the current research on antimicrobial peptides is provided, currently being developed for the treatment of recalcitrant bacterial infections, the synergies of AMPs and antibiotics, and the activity of AMP against biofilm.
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