Biosensors for detection of pathogenic bacteria

TL;DR: This edition of 'Flow Cytometry and Sorting' is ambitious, comprising 39 chapters written by 70 authors expert in their area of flow cytometry, and the chapters are divided into eight parts.

TL;DR: A review of the most commonly used biosensor systems based on their transducer properties, which include surface plasmon resonance (SPR), amperometric, potentiometric, and acoustic wave sensors and their applications for the detection of pathogens in food and water is presented in this paper.

Abstract: The incidence of foodborne diseases has increased over the years and resulted in major public health problem globally. Foodborne pathogens can be found in various foods and it is important to detect foodborne pathogens to provide safe food supply and to prevent foodborne diseases. The conventional methods used to detect foodborne pathogen are time consuming and laborious. Hence, a variety of methods have been developed for rapid detection of foodborne pathogens as it is required in many food analyses. Rapid detection methods can be categorized into nucleic acid-based, biosensor-based and immunological-based methods. This review emphasizes on the principles and application of recent rapid methods for the detection of foodborne bacterial pathogens. Detection methods included are simple polymerase chain reaction (PCR), multiplex PCR, real-time PCR, nucleic acid sequence-based amplification (NASBA), loop-mediated isothermal amplification (LAMP) and oligonucleotide DNA microarray which classified as nucleic acid-based methods; optical, electrochemical and mass-based biosensors which classified as biosensor-based methods; enzyme-linked immunosorbent assay (ELISA) and lateral flow immunoassay which classified as immunological-based methods. In general, rapid detection methods are generally time-efficient, sensitive, specific and labor-saving. The developments of rapid detection methods are vital in prevention and treatment of foodborne diseases.

TL;DR: A review about the potential application of biosensor technology in drink and food industries, its current situation and potential is presented.

TL;DR: HUS is relatively common in Canadian children younger than 5 years, and is strongly associated with E. coli O157:H7 infection, and reasons for the striking regional variation in the incidence are unexplained.

TL;DR: A biosensor strategy for rapid amperometric detection of organisms was investigated in which a redox mediator was entrapped within liposomes, and it was found that selective release of mediator by haemolytic bacteria, followed by signal generation, confers differentiated species.

TL;DR: High cell concentrations of more than 10(8) cfu/g of S. putrefaciens were required for production of detectable off-odours and it was concluded that this organism is without importance for spoilage of packed cod.

TL;DR: The Hartman interferometer addresses the challenge of creating a simple, low-cost configuration for multianalyte testing by relying on linearly polarized light and a planar waveguide format, thereby avoiding the problems associated with circular polarization and channel waveguides.