Journal Article10.1021/acssensors.2c01730
Biosensor Optimization Using a Förster Resonance Energy Transfer Pair Based on mScarlet Red Fluorescent Protein and an mScarlet-Derived Green Fluorescent Protein.
10
TL;DR: In this paper , an mScarlet-derived green fluorescent protein (designated as mWatermelon) was used as a FRET donor to the red fluorescent protein m Scarlet-I as an acceptor.
read more
Abstract: Genetically encoded biosensors based on Förster resonance energy transfer (FRET) are indispensable tools for monitoring biochemical changes in cells. Green and red fluorescent protein-based FRET pairs offer advantages over the classically employed cyan and yellow fluorescent protein pairs, such as better spectral separation, lower phototoxicity, and less autofluorescence. Here, we describe the development of an mScarlet-derived green fluorescent protein (designated as mWatermelon) and its use as a FRET donor to the red fluorescent protein mScarlet-I as a FRET acceptor. We tested the functionality of this FRET pair by engineering biosensors for the detection of protease activity, Ca2+, and K+. Furthermore, we described a strategy to enhance the FRET efficiency of these biosensors by modulating the intramolecular association between mWatermelon and mScarlet-I.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Advances in FRET‐based biosensors from donor‐acceptor design to applications
Wen‐Chao Yang,Shuang‐Yu Li,Shengnan Ni,Guozhen Liu +3 more
TL;DR: The recent advance in the discovery of FRET biosensors using donor‐acceptor dye combinations is described and they are classified based on different types of analytes, such as mall molecules, proteins, enzymes, nucleic acids and metal ions.
26
Principles and applications of green fluorescent protein-based biosensors: a mini-review.
TL;DR: Green fluorescent proteins (GFPs) are useful and essential biomolecules that have revolutionized biosensor research as mentioned in this paper and have extensively utilized GFPs for designing fluorescence biosensors due to their intrinsic fluorescence, high stability, and ability to undergo permutation or mutation.
12
Fluorescent Protein-Based Sensors for Detecting Essential Metal Ions across the Tree of Life.
Gary C. Jensen,Makena K. Janis,Hazel N Nguyen,Ogonna W David,Melissa L. Zastrow +4 more
TL;DR: The aim of this Perspective is to provide an updated and critical view of the design and use of fluorescent protein-based sensors for detecting essential metal ions in various organisms.
5
Exploring the landscape of FRET-based molecular sensors: Design strategies and recent advances in emerging applications
Neha Soleja,Mohd. Mohsin +1 more
TL;DR: This review explores FRET-based molecular sensors, discussing design strategies, recent advances, and emerging applications in real-time bioimaging, including multiplexing capabilities and multi-step protocols for dual/multi-analyte detections in chemical biology.
5
Red fluorescent protein dimer for singlet oxygen (1O2)/ superoxide radical (O2•−) simultaneously mediated photodynamic therapy and two-photon fluorescence imaging
Weilong Li,Ying Qian +1 more
TL;DR: Researchers synthesized a red fluorescent protein dimer (DAPFP-lyso) for simultaneous photodynamic therapy and two-photon fluorescence imaging, exhibiting enhanced singlet oxygen and superoxide radical production, and effective two-photon absorption for vivid imaging in zebrafish.
4
References
Calcium signalling: dynamics, homeostasis and remodelling
TL;DR: The Ca2+-signalling toolkit is used to assemble signalling systems with very different spatial and temporal dynamics and has a direct role in controlling the expression patterns of its signalling systems that are constantly being remodelled in both health and disease.
Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin
Atsushi Miyawaki,Juan Llopis,Roger Heim,J. Michael McCaffery,Joseph A. Adams,Mitsuhiko Ikura,Mitsuhiko Ikura,Roger Y. Tsien +7 more
TL;DR: New fluorescent indicators for Ca2+ that are genetically encoded without cofactors and are targetable to specific intracellular locations are constructed and dubbed ‘cameleons’.
A monomeric red fluorescent protein
Robert E. Campbell,Oded Tour,Amy E. Palmer,Paul Steinbach,Geoffrey S. Baird,David A. Zacharias,Roger Y. Tsien +6 more
TL;DR: This work presents the stepwise evolution of DsRed to a dimer and then either to a genetic fusion of two copies of the protein, i.e., a tandem dimer, or to a true monomer designated mRFP1 (monomeric red fluorescent protein).
A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum.
Nathan C. Shaner,Gerard G. Lambert,Andrew Chammas,Yuhui Ni,Paula J. Cranfill,Michelle A. Baird,Brittney R. Sell,John R. Allen,Richard O. Day,Maria Israelsson,Michael W. Davidson,Jiwu Wang +11 more
TL;DR: In this article, a monomeric yellow green fluorescent protein, mNeonGreen, derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum, was described.
An Expanded Palette of Genetically Encoded Ca2+ Indicators
Yongxin Zhao,Satoko Araki,Jiahui Wu,Takayuki Teramoto,Yu-Fen Chang,Masahiro Nakano,Ahmed S. Abdelfattah,Manabi Fujiwara,Takeshi Ishihara,Takeharu Nagai,Takeharu Nagai,Robert E. Campbell +11 more
TL;DR: This series enables improved single-color Ca2+ imaging in neurons and transgenic Caenorhabditis elegans and develops blue, improved green, and red intensiometric indicators, as well as an emission ratiometric indicator with an 11,000% ratio change.