Open AccessJournal Article
Biochip development for polymorphism analysis in biotransformation system genes
Glotov aS,T. V. Nasedkina,Ivashchenko Te,R A Iurasov,Sergey Surzhikov,S. V. Pan'kov,Alexander V. Chudinov,Vladislav S Baranov,Alexander S. Zasedatelev +8 more
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TL;DR: The efficiency of using the method of allele-specific hybridization with biochips for identification of individual genetic polymorphism is shown and it is believed that the bioch chips can be also a convenient tool in pharmacogenetics researches.
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Abstract: Large-scale population researches, diagnostics of genetic predisposition to multifactorial diseases, screening of the polymorphic loci associated with individual sensitivity to pharmaceutical preparations, require the development of effective, exact and rapid methods of analysis for detection of many mutations simultaneously. One of the most perspective methods to solve these problems is a method of allele-specific hybridization with biochips. Taking the analysis of mutations in genes CYP1A1, CYP2D6, GSTM1, GSTT1, NAT2, CYP2C9, CYP2C19 and MTHFR as an example we showed the efficiency of using the approach for identification of individual genetic polymorphism. We believe that the biochips can be also a convenient tool in pharmacogenetics researches.
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Citations
Microarray-Based Detection of CYP1A1, CYP2C9, CYP2C19, CYP2D6, GSTT1, GSTM1, MTHFR, MTRR, NQO1, NAT2, HLA-DQA1, and AB0 Allele Frequencies in Native Russians
O. A. Gra,Olga N. Mityaeva,I. N. Berdichevets,Zhanna Kozhekbaeva,D. O. Fesenko,Olga Kurbatova,I. V. Goldenkova-Pavlova,Tatyana V. Nasedkina +7 more
TL;DR: The HLA-DQA1 and AB0 allele frequencies were used to estimate forensic population parameters for these loci, and data were used in comparative studies to determine predisposition to tuberculosis, lymphoma, and leukemia in adults and to childhood acute leukemia.
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Diagnostic Microarrays in Hematologic Oncology
Tatyana V. Nasedkina,Natalia A. Guseva,O. A. Gra,Olga N. Mityaeva,Alexander V. Chudinov,Alexander S. Zasedatelev +5 more
TL;DR: Gel-based microarrays (biochips) consist of nanoliter gel drops on a hydrophobic surface with different immobilized biopolymers (primarily nucleic acids and proteins) and have a high immobilization capacity of the gel, which allow the use of simple, portable detection systems.
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Analysis of Point Mutations of the BRCA1 Gene by Hybridization with Hydrogel Microarrays
O. E. Fedorova,Olga Sinicka,Laima Tihomirova,Ya. V. Visnevskaya,Alexander S. Zasedatelev,T. V. Nasedkina +5 more
TL;DR: The results demonstrated the advantage of employing the microarray in analyzing BRCA1 mutations and demonstrated the need to select an individual treatment strategy for BC prognosis and prevention of disease recurrence.
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Genome Paths A Way to Personalized and Predictive Medicine
TL;DR: The concept of a genetic pass as an individual DNA databank reflecting inherited human predisposition to different complex and monogenic disorders, with special emphasis on its present state, and the numerous difficulties related to the practical implementation of personalized medicine are outlined.
Biochip analysis of BRCA1/2 and CHEK2 common mutations in ovarian cancer and primary multiple tumors involving the ovaries (Russian population)
O. E. Fedorova,L. N. Lyubchenko,Yu. G Payanidi,Tatiana P. Kazubskaya,F. A. Amosenko,R. F. Garkavtseva,Alexander S. Zasedatelev,Tatyana V. Nasedkina +7 more
TL;DR: Using biochip technology, the frequencies of eight BRCA1/2 and CHEK2 mutations were studied in Russian women with OC, including 68 patients with organ-specific OC and 19 with primary multiple tumors involving the ovaries, where the last one was most common in OC.
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