Journal Article10.1038/NRMICRO2315
Bacteriophage resistance mechanisms.
2.2K
TL;DR: This Review highlights the most important antiviral mechanisms of bacteria as well as the counter-attacks used by phages to evade these systems.
read more
Abstract: Phages are now acknowledged as the most abundant microorganisms on the planet and are also possibly the most diversified. This diversity is mostly driven by their dynamic adaptation when facing selective pressure such as phage resistance mechanisms, which are widespread in bacterial hosts. When infecting bacterial cells, phages face a range of antiviral mechanisms, and they have evolved multiple tactics to avoid, circumvent or subvert these mechanisms in order to thrive in most environments. In this Review, we highlight the most important antiviral mechanisms of bacteria as well as the counter-attacks used by phages to evade these systems.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
The highly diverse antiphage defence systems of bacteria.
TL;DR: Findings reveal that bacterial immunity is much more complex than previously thought and the ecological impact of their diversity is revealed.
206
The adaptation of temperate bacteriophages to their host genomes.
TL;DR: The results show how natural selection for seamless integration of prophages in the chromosome shapes the evolution of the bacterium and the phage.
206
Evaluation of methods to purify virus-like particles for metagenomic sequencing of intestinal viromes
TL;DR: The CsCl density gradient centrifugation method, which is frequently used for VLP purification, was most efficient in removing host derived DNA, but also showed strong discrimination against specific phages and showed a lower reproducibility of quantitative results.
Bacteriophage therapy against Pseudomonas aeruginosa biofilms: a review.
Zahra Chegini,Amin Khoshbayan,Majid Taati Moghadam,Iman Farahani,Parham Jazireian,Aref Shariati +5 more
TL;DR: The use of phage therapy for inhibition of P. aeruginosa biofilm in clinical and in vitro studies is discussed to identify different aspects of this treatment for broader use.
Strong bias in the bacterial CRISPR elements that confer immunity to phage
David Paez-Espino,Wesley Morovic,Christine L. Sun,Brian C. Thomas,Ken-ichi Ueda,Buffy Stahl,Rodolphe Barrangou,Jillian F. Banfield +7 more
TL;DR: Nearly all phage locations targeted by the acquired spacers have a proto-spacer adjacent motif (PAM), indicating PAMs are involved in spacer acquisition and reflect selection for specific spacers based on location and effectiveness.
References
CRISPR provides acquired resistance against viruses in prokaryotes
Rodolphe Barrangou,Christophe Fremaux,Hélène Deveau,Melissa Richards,Patrick Boyaval,Sylvain Moineau,Dennis A. Romero,Philippe Horvath +7 more
TL;DR: It is found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences, and CRISPR provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.
Small CRISPR RNAs guide antiviral defense in prokaryotes
Stan J. J. Brouns,Matthijs M. Jore,Magnus Lundgren,Edze R. Westra,Rik J. H. Slijkhuis,Ambrosius P. Snijders,Mark J. Dickman,Kira S. Makarova,Eugene V. Koonin,John van der Oost +9 more
TL;DR: The results demonstrate that the formation of mature guide RNAs by the CRISPR RNA endonuclease subunit of Cascade is a mechanistic requirement for antiviral defense.
CRISPR/Cas, the Immune System of Bacteria and Archaea
TL;DR: Clustered regularly interspaced short palindromic repeats (CRISPR) form peculiar genetic loci, which provide acquired immunity against viruses and plasmids by targeting nucleic acid in a sequence-specific manner.
2.5K
Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product.
TL;DR: Neither the isozyme-converting activity nor labeled Iap proteins were detected in the osmotic-shock fluid of cells carrying a multicopy iap plasmid, and the Iap protein seems to be associated with the membrane.
2.2K
Intervening Sequences of Regularly Spaced Prokaryotic Repeats Derive from Foreign Genetic Elements
TL;DR: It is shown that CRISPR spacers derive from preexisting sequences, either chromosomal or within transmissible genetic elements such as bacteriophages and conjugative plasmids, implying a relationship betweenCRISPR and immunity against targeted DNA.
2.1K