Book Chapter10.1385/1-59259-809-9:353
Automated NMR structure calculation with CYANA.
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TL;DR: This chapter gives an introduction to automated nuclear magnetic resonance (NMR) structure calculation with the program CYANA.
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Abstract: This chapter gives an introduction to automated nuclear magnetic resonance (NMR) structure calculation with the program CYANA. Given a sufficiently complete list of assigned chemical shifts and one or several lists of cross-peak positions and columns from two-, three-, or four-dimensional nuclear Overhauser effect spectroscopy (NOESY) spectra, the assignment of the NOESY cross-peaks and the three-dimensional structure of the protein in solution can be calculated automatically with CYANA.
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Citations
Improving NMR Protein Structure Quality by Rosetta Refinement: A Molecular Replacement Study
Theresa Ramelot,Srivatsan Raman,Alexandre P. Kuzin,Rong Xiao,Li Chung Ma,Thomas Acton,John F. Hunt,Gaetano T. Montelione,David Baker,Michael A. Kennedy +9 more
TL;DR: Inclusion of nonbivalent hydrogen bonds identified from Rosetta structures as additional restraints in the structure calculation results in NMR structures with improved MR performance, and the superior hydrogen‐bond network in Rosetta‐refined structures demonstrates that correct identification of hydrogen bonds should be a critical goal of NMR structure refinement.
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Structural basis of mitochondrial dysfunction in response to cytochrome c phosphorylation at tyrosine 48.
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TL;DR: It is reported that a phosphomimetic mutant of cy tochrome c exhibits enhanced dynamics, which could be responsible for the observed differences in cytochrome c functionality in oxidative stress and cell death, and becomes a target for further development of robust therapeutic approaches.
Zn-regulated GTPase metalloprotein activator 1 modulates vertebrate zinc homeostasis
Andy Weiss,Caitlin C. Murdoch,Katherine A. Edmonds,Matthew R. Jordan,Andrew J. Monteith,Y. Randika Perera,Aslin M. Rodríguez Nassif,Amber M. Petoletti,William N. Beavers,Matthew J. Munneke,Sydney Drury,Evan S. Krystofiak,Kishore Thalluri,Hongwei Wu,Angela Kruse,Richard D. DiMarchi,Richard M. Caprioli,Jeffrey M. Spraggins,Walter J. Chazin,David P. Giedroc,Eric P. Skaar +20 more
TL;DR: In this article , the Zn-regulated GTPase metalloprotein activator (ZNG1) family has been found to have an important role for intracellular Zn trafficking.
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Fusion to a highly stable consensus albumin binding domain allows for tunable pharmacokinetics
TL;DR: The engineering of a novel albumin binding domain (ABD) three-helix bundle protein, called ABDCon, is described, which binds human, monkey and mouse serum albumins with affinity as high as 61 pM and is highly stable.
NMR solution structure of the N-terminal domain of hERG and its interaction with the S4-S5 linker.
TL;DR: NMR titration experiment demonstrated that residues from 88 to 94 in NTD are important for the molecular interaction with the peptide derived from the S4-S5 linker as well as for the protein-protein or protein-membrane interactions.
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