Automated Analysis of Single-Molecule Photobleaching Data by Statistical Modeling of Spot Populations.
Clarissa Liesche,Clarissa Liesche,Kristin S. Grußmayer,Michael Ludwig,Stefan Wörz,Stefan Wörz,Karl Rohr,Karl Rohr,Dirk-Peter Herten,Joël Beaudouin,Joël Beaudouin,Roland Eils,Roland Eils +12 more
TL;DR: This study proposes a new approach, to the knowledge, to determine the fluorophore number that correlates the intensity decay of a population of molecule complexes with the decay of the number of visible complexes, and is fully automated, robust to noise, and does not require the counting of photobleaching events.
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About: This article is published in Biophysical Journal. The article was published on 01 Dec 2015. and is currently open access. The article focuses on the topics: Photobleaching & Fluorophore.
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Live-Cell Imaging Shows Uneven Segregation of Extrachromosomal DNA Elements and Transcriptionally Active Extrachromosomal DNA Hubs in Cancer
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Single Molecule Light Field Microscopy
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TL;DR: In this paper, single molecule light field microscopy (SMLFM) is proposed to image a single fluorophore from different angles by segmenting the back focal plane of a microscope objective with an array of microlenses.
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Exon-independent recruitment of SRSF1 is mediated by U1 snRNP stem-loop 3
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