ATR-FTIR spectrum analysis of saliva samples from COVID-19 positive patients.
Adriana Martinez-Cuazitl,Gustavo Jesus Vazquez-Zapien,Miguel Sanchez-Brito,Jorge H. Limón-Pacheco,Melissa Guerrero-Ruiz,Francisco Garibay-Gonzalez,R. Delgado-Macuil,Maria G. Garcia de Jesus,Miguel A. Corona-Perezgrovas,Armando Pereyra-Talamantes,Monica Maribel Mata-Miranda +10 more
TL;DR: Specific salivary vibrational modes analyzed by attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy were established, allowing the detection for COVID-19 using an MLRM, once it helps to reduce the number of variables, which could be helpful in the future development of diagnostic devices in a faster and cheaper way.
read more
Abstract: The coronavirus disease 2019 (COVID-19) is the latest biological hazard for the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Even though numerous diagnostic tests for SARS-CoV-2 have been proposed, new diagnosis strategies are being developed, looking for less expensive methods to be used as screening. This study aimed to establish salivary vibrational modes analyzed by attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy to detect COVID-19 biological fingerprints that allow the discrimination between COVID-19 and healthy patients. Clinical dates, laboratories, and saliva samples of COVID-19 patients (N = 255) and healthy persons (N = 1209) were obtained and analyzed through ATR-FTIR spectroscopy. Then, a multivariate linear regression model (MLRM) was developed. The COVID-19 patients showed low SaO2, cough, dyspnea, headache, and fever principally. C-reactive protein, lactate dehydrogenase, fibrinogen, D-dimer, and ferritin were the most important altered laboratory blood tests, which were increased. In addition, changes in amide I and immunoglobulin regions were evidenced in the FTIR spectra analysis, and the MLRM showed clear discrimination between both groups. Specific salivary vibrational modes employing ATR-FTIR spectroscopy were established; moreover, the COVID-19 biological fingerprint in saliva was characterized, allowing the COVID-19 detection using an MLRM, which could be helpful for the development of new diagnostic devices.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Advances in Electrochemical Biosensors Based on Nanomaterials for Protein Biomarker Detection in Saliva
TL;DR: In this article , the role of nanomaterials to boost the analytical performance and increase the reliability of the test in human saliva samples is discussed, and the critical factors for further modernization of the Nanomaterial-based electrochemical sensors, envisaging the development and implementation of next generation sample-in-answer-out systems.
57
Attenuated total reflection-Fourier transform infrared spectroscopy: a universal analytical technique with promising applications in forensic analyses
TL;DR: Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) is a modern and universal analytical technique capable of fingerprint recognition of the analyte using minimal amount of the test sample as mentioned in this paper .
Saliva as a sample type for SARS-CoV-2 detection: implementation successes and opportunities around the globe
Emily R Tobik,Lily Kitfield-Vernon,Russell J Thomas,Sydney A Steel,Steph H Tan,Orchid M. Allicock,Brittany L. Choate,Sumaira Akbarzada,Anne L. Wyllie +8 more
TL;DR: Here, localities that have demonstrated success with saliva-based SARS-CoV-2 testing approaches are reviewed and can serve as models for transforming concepts into globally-implemented best practices.
30
Electrochemical Immunosensors Based on Zinc Oxide Nanorods for Detection of Antibodies Against SARS-CoV-2 Spike Protein in Convalescent and Vaccinated Individuals
Freddy Núñez,Ana Cristina Honorato De Castro,Vivian L. de Oliveira,Ariane Cassia de Lima,Jamille R Oliveira,G. X. de Medeiros,Greyce Luri Sasahara,Keity Souza Santos,Alexandre J. C. Lanfredi,Wendel A. Alves +9 more
TL;DR: An impedimetric biosensor based on the immobilization of SARS-CoV-2 recombinant trimeric spike protein on zinc oxide nanorod (ZnONR)-modified fluorine-doped tin oxide substrates for COVID-19 serology testing shows excellent potential as a tool for the population’s assessment and monitoring of seroconversion and seroprevalence.
25
Current trends in COVID-19 diagnosis and its new variants in physiological fluids: Surface antigens, antibodies, nucleic acids, and RNA sequencing
Menna Mostafa,Ahmed Barhoum,Ekin Sehit,Hossam Eid Gewaid,Eslam S. Mostafa,Mohamed Omran,Mohga S Abdalla,Fatehy M. Abdel-Haleem,Zeynep Altintas,Robert J. Forster +9 more
TL;DR: A comprehensive overview of the current techniques developed for the detection of SARS-CoV-2 in various body fluids (e.g., blood, urine, feces, saliva, tears, and semen) and considers the mutations (i.e., Alpha, Beta, Gamma, Delta, Omicron) is provided in this paper .
23
References
Clinical Characteristics of 138 Hospitalized Patients With 2019 Novel Coronavirus-Infected Pneumonia in Wuhan, China.
Dawei Wang,Bo Hu,Chang Hu,Fangfang Zhu,Xing Liu,Jing Zhang,Binbin Wang,Hui Xiang,Zhenshun Cheng,Yong Xiong,Yan Zhao,Yirong Li,Xinghuan Wang,Zhiyong Peng +13 more
TL;DR: The epidemiological and clinical characteristics of novel coronavirus (2019-nCoV)-infected pneumonia in Wuhan, China, and hospital-associated transmission as the presumed mechanism of infection for affected health professionals and hospitalized patients are described.
18.7K
Detection of SARS-CoV-2 in Different Types of Clinical Specimens.
TL;DR: Results of PCR and viral RNA testing for SARS-CoV-2 in bronchoalveolar fluid, sputum, feces, blood, and urine specimens from patients with COVID-19 infection in China are described to identify possible means of non-respiratory transmission.
Characteristics of SARS-CoV-2 and COVID-19
TL;DR: The basic virology of SARS-CoV-2 is described, including genomic characteristics and receptor use, highlighting its key difference from previously known coronaviruses.