Artificial intelligence-assisted identification and quantification of osteoclasts
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TL;DR: Estimation of Oc.S/BS by the new AI-assisted method was considerably less time-consuming, while still providing similar results to the conventional manual method, and a detailed step-by-step guide for the AI-based method is presented.
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About: This article is published in MethodsX. The article was published on 01 Jan 2021. and is currently open access.
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Citations
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OC_Finder: Osteoclast Segmentation, Counting, and Classification Using Watershed and Deep Learning
TL;DR: OC_Finder, a fully automated system for identifying osteoclasts in microscopic images, suggests that deep learning is a useful tool to perform prompt and accurate unbiased classification and detection of specific cell types in microscopic pictures.
Contemporary Advances in Computer-Assisted Bone Histomorphometry and Identification of Bone Cells in Culture
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TL;DR: An overview of contemporary methods utilizing specialized computer software to perform bone histomorphometry or identification of bone cells in culture and a substantial need for more validation studies to evaluate the accuracy of the new computational methods to the manual and conventional analyses.
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References
Osteoclasts: New Insights
Feng Xu,Steven L. Teitelbaum +1 more
TL;DR: New advances in the understanding of the mechanisms of osteoclast differentiation and function are discussed, including c-Fms and RANK signaling have been shown to regulate bone resorption by cross-talking with those activated by integrin αvβ3.
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An Optimized Approach to Perform Bone Histomorphometry.
Deeksha Malhan,Matthias Muelke,Matthias Muelke,Sebastian Rosch,Annemarie B. Schaefer,Felix Merboth,David Weisweiler,David Weisweiler,Christian Heiss,Christian Heiss,Ignacio Arganda-Carreras,Thaqif El Khassawna +11 more
TL;DR: The adaptation of Trainable Weka Segmentation plugin of ImageJ to allow fast evaluation of bone parameters (trabecular area, osteoid area) to diagnose bone related diseases and two different scripts developed for bone histomorphometry can be optimized globally for other histological samples.
Optimization of the tartrate-resistant acid phosphatase detection by histochemical method
TL;DR: The present study determined the optimal temperature for identification of TRACP in activated osteoclasts in undecalcified bone biopsies embedded in methylmethacrylate and found that the best results were obtained with temperatures between 60°C and 70°C.
Formation and function of the ruffled border in osteoclasts
TL;DR: Osteoclasts are multinucleated hematopoietic cells specialised for bone resorption, which has unusual features more similar to endosomal/lysosomal membranes.
The Efficacy of PTH and Abaloparatide to Counteract Immobilization-Induced Osteopenia Is in General Similar.
TL;DR: In a mole-to-mole comparison the efficacy of PTH and ABL is similar in counteracting immobilization-induced reduction in bone mineral density, deterioration in trabecular microarchitecture, and decrease in bone strength.