Journal Article10.1210/JCEM-67-6-1186
Arginine stimulates growth hormone secretion by suppressing endogenous somatostatin secretion.
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TL;DR: The results indicate that Arg administered with GHRH led to higher serum GH levels than did a maximally stimulatory dose of G HRH or Arg alone, and the stimulatory effects of Arg are mediated by suppression of endogenous somatostatin secretion.
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Abstract: To determine how arginine (Arg) stimulates GH secretion, we investigated its interaction with GHRH in vivo and in vitro. Six normal men were studied on four occasions: 1) Arg-TRH, 30 g arginine were administered in 500 mL saline in 30 min, followed by an injection of 200 micrograms TRH; 2) GHRH-Arg-TRH, 100 micrograms GHRH-(1-44) were given iv as a bolus immediately before the Arg infusion, followed by 200 micrograms TRH, iv; 3) GHRH test, 100 micrograms GHRH were given as an iv bolus; and 4) TRH test, 200 micrograms TRH were given iv as a bolus dose. Blood samples were collected at 15-min intervals for 30 min before and 120 min after the start of each infusion. Anterior pituitary cells from rats were coincubated with Arg (3, 6, 15, 30, and 60 mg/mL) and GHRH (0.05, 1, 5, and 10 nmol/L) for a period of 3 h. Rat GH was measured in the medium. After Arg-TRH the mean serum GH concentration increased significantly from 0.6 to 23.3 +/- 7.3 (+/- SE) micrograms/L at 60 min. TRH increased serum TSH and PRL significantly (maximum TSH, 11.1 +/- 1.8 mU/L; maximum PRL, 74.6 +/- 8.4 micrograms/L). After GHRH-Arg-TRH, the maximal serum GH level was significantly higher (72.7 +/- 13.4 micrograms/L) than that after Arg-TRH alone, whereas serum TSH and PRL increased to comparable levels (TSH, 10.2 +/- 3.0 mU/L; PRL, 64.4 +/- 13.6 micrograms/L). GHRH alone increased serum GH to 44.9 +/- 9.8 micrograms/L, significantly less than when GHRH, Arg, and TRH were given. TRH alone increased serum TSH to 6.6 +/- 0.6 mU/L, significantly less than the TSH response to Arg-TRH. The PRL increase after TRH only also was lower (47.2 +/- 6.8 micrograms/L) than the PRL response after Arg-TRH. In vitro Arg had no effect on basal and GHRH-stimulated GH secretion. Our results indicate that Arg administered with GHRH led to higher serum GH levels than did a maximally stimulatory dose of GHRH or Arg alone. The serum TSH response to Arg-TRH also was greater than that to TRH alone. We conclude that the stimulatory effects of Arg are mediated by suppression of endogenous somatostatin secretion.
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Citations
Interaction of L-dopa and GHRH on GH secretion in normal men
TL;DR: The results show that the combination of L-dopa with G HRH leads to the same GH response as GHRH only, and the inhibition of the TSH and PRL response to TRH after L-Dopa administration, however, is due to the direct action of the L- dopa metabolite dopamine on the anterior pituitary.
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Stimulation of Growth Hormone Release in Man by the Potent D2-Dopamine Agonist CV 205-502: Comparison of Responses to Intravenous and Oral Administration
TL;DR: It is well known that dopaminergic agents are stimulators of GH release in man, and although responses are sometimes unreliable, oral L-dopa and iv dopamine have frequently been employed in the evaluation of GH-deficient states and a typical dose-response relationship was found.
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Myalgia and Hematuria in Association with Clonidine and Arginine Administration for Growth Hormone Stimulation Tests.
Meghan Glibbery,Meghan Glibbery,Adam J Fleming,Adam J Fleming,Rahul Chanchlani,Rahul Chanchlani,Olufemi Ajani,Norma Marchetti,Alexa Marr,Alexa Marr,M. Constantine Samaan +10 more
TL;DR: This case highlights an unusual combination of adverse reactions to clonidine and arginine in children undergoing GH stimulation testing to assess for GHD, and Pediatric endocrinologists need to be aware of the potential for these side effects.
GH secretion in Prader-Labhard-Willi syndrome: somatotrope responsiveness to GHRH is enhanced by arginine but not by pyridostigmine.
TL;DR: Results demonstrate that in Prader-Labhard-Willi Syndrome the low somatotrope responsiveness to GHRH is not enhanced by cholinergic potentiation while it is increased by arginine.
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Comparison of the Effects of Ornithine and Arginine on the Brain Protein Synthesis Rate in Young Rats
Shoko Suzumura,Kazuyo Tujioka,Takashi Yamada,Hidehiko Yokogoshi,Saori Akiduki,Yukihiro Hishida,Kazumi Tsutsui,Kazutoshi Hayase +7 more
TL;DR: The results suggest that the treatment with arginine is likely to increase the concentrations of GH and the rate of brain protein synthesis in rats, and that the effects ofArginine on brain protein synthesized and GH concentration were lower than that of ornithine.
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References
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TL;DR: A dose of 1 micrograms/kg GRF-44 is safe and effective, and would appear to be a reasonable choice for use in studying GH responses in normal subjects of other ages and in patients with disorders of GH secretion.
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Effect of growth hormone (GH)-releasing hormone (GRH) on plasma GH in relation to magnitude and duration of GH deficiency in 26 children and adults with isolated GH deficiency or multiple pituitary hormone deficiencies: evidence for hypothalamic GRH deficiency.
Elizabeth A. Schriock,Robert H. Lustig,Stephen M. Rosenthal,Selna L. Kaplan,Melvin M. Grumbach +4 more
TL;DR: The GH responses to GRH suggest that the majority of patients with isolated GHD as well as those with multiple hypothalamic-pituitary hormone deficiencies have deficiency of hypothalamic GRH .
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The arginine provocative test: an aid in the diagnosis of hyposomatotropism.
TL;DR: Comparisons have been made of the secretion of growth hormone (HGH) following the induction of hypoglycemia with insulin and that induced by arginine infusion to determine the reliability of the latter procedure in the diagnosis of disturbances in growth hormone secretion in children and adult patients.
142
Growth hormone (GH) release in response to GH-releasing hormone in man is 3-fold enhanced by galanin.
TL;DR: The effect of GHRH in a dose (120 μg) thought to produce a maximal GH response was compared with the GH response to insulin-induced hypoglycemia, iv infusion of the hypothalamic neuropeptide galanin (40 pmol/kg·min for 40 min), and a combination of gHRH and Galanin in normal men as mentioned in this paper.
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L-dopa stimulates release of hypothalamic growth hormone-releasing hormone in humans.
Kazuo Chihara,Yoichi Kashio,Tetsuya Kita,Yasuhiko Okimura,Hidesuke Kaji,Hiromi Abe,Takuo Fujita +6 more
TL;DR: A sensitive RIA for human GH-releasing hormone-(1-44)-NH2 [hGHRH-( 1-44]-NH2] was developed which allows its measurement in human plasma extracts.
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