Arabidopsis stomatal initiation is controlled by MAPK-mediated regulation of the bHLH SPEECHLESS.
TL;DR: It is found that a unique domain in a basic helix-loop-helix (bHLH) stomatal initiating factor, SPEECHLESS, renders it a MAPK phosphorylation target in vitro and modulates its function in vivo.
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Abstract: Stomata, epidermal structures that modulate gas exchange between plants and the atmosphere, play critical roles in primary productivity and the global climate. Positively acting transcription factors and negatively acting mitogen-activated protein kinase (MAPK) signaling control stomatal development in Arabidopsis; however, it is not known how the opposing activities of these regulators are integrated. We found that a unique domain in a basic helix-loop-helix (bHLH) stomatal initiating factor, SPEECHLESS, renders it a MAPK phosphorylation target in vitro and modulates its function in vivo. MAPK cascades modulate a diverse set of activities including development, cell proliferation, and response to external stresses. The coupling of MAPK signaling to SPEECHLESS activity provides cell type specificity for MAPK output while allowing the integration of multiple developmental and environmental signals into the production and spacing of stomata.
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Citations
Regulation of Cell Proliferation in the Stomatal Lineage by the Arabidopsis MYB FOUR LIPS via Direct Targeting of Core Cell Cycle Genes
Zidian Xie,Eun-Kyoung Lee,Eun-Kyoung Lee,Jessica R. Lucas,Kengo Morohashi,Dongmei Li,James A. H. Murray,Fred D. Sack,Fred D. Sack,Erich Grotewold +9 more
TL;DR: It is proposed that FLP and MYB88 together integrate patterning with the control of cell cycle progression and terminal differentiation through multiple and direct cell cycle targets.
Differentiation of Arabidopsis guard cells: analysis of the networks incorporating the basic helix-loop helix transcription factor, FAMA
TL;DR: This work profiles transcriptional changes in response to inducible expression of Arabidopsis (Arabidopsis thaliana) FAMA, a basic helix-loop-helix protein whose actions during the final stage in stomatal development regulate both cell division and cell fate.
Changes in intracellular NAD status affect stomatal development in an abscisic acid-dependent manner.
Elias Feitosa-Araujo,Elias Feitosa-Araujo,Paula da Fonseca-Pereira,Mateus M Pena,David B. Medeiros,Leonardo Perez de Souza,Takuya Yoshida,Andreas P.M. Weber,Wagner L. Araújo,Alisdair R. Fernie,Markus Schwarzländer,Adriano Nunes-Nesi +11 more
TL;DR: It is found that NAD negatively impacts stomatal development in cotyledons of Arabidopsis thaliana, demonstrating that intracellular NAD+ homeostasis as set by synthesis, breakdown and transport is essential for normal stomatic development, and provide a link between central metabolism, hormone signaling and developmental plasticity.
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