Arabidopsis stomatal initiation is controlled by MAPK-mediated regulation of the bHLH SPEECHLESS.
TL;DR: It is found that a unique domain in a basic helix-loop-helix (bHLH) stomatal initiating factor, SPEECHLESS, renders it a MAPK phosphorylation target in vitro and modulates its function in vivo.
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Abstract: Stomata, epidermal structures that modulate gas exchange between plants and the atmosphere, play critical roles in primary productivity and the global climate. Positively acting transcription factors and negatively acting mitogen-activated protein kinase (MAPK) signaling control stomatal development in Arabidopsis; however, it is not known how the opposing activities of these regulators are integrated. We found that a unique domain in a basic helix-loop-helix (bHLH) stomatal initiating factor, SPEECHLESS, renders it a MAPK phosphorylation target in vitro and modulates its function in vivo. MAPK cascades modulate a diverse set of activities including development, cell proliferation, and response to external stresses. The coupling of MAPK signaling to SPEECHLESS activity provides cell type specificity for MAPK output while allowing the integration of multiple developmental and environmental signals into the production and spacing of stomata.
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Citations
Targeting editing of tomato SPEECHLESS cis-regulatory regions generates plants with altered stomatal density in response to changing climate conditions
I. Nir,Alanta Budrys,N. K. Smoot,Joel M. Erberich,Dominique C. Bergmann +4 more
TL;DR: By precise gene editing of a core stomatal development regulator gene in tomato, new alleles with enhanced or dampened responses to light and temperature cues are generated and combined with live imaging of development, it is shown how this could lead to better climate-adapted varieties.
3
MUTE Directly Orchestrates Cell State Switch and the Single Symmetric Division to Create Stomata
Soon-Ki Han,Xingyun Qi,Kei Sugihara,Jonathan H. Dang,Takaho A. Endo,Kristen L. Miller,Eun-Deok Kim,Takashi Miura,Keiko U. Torii +8 more
TL;DR: In this article, the authors report the complete inventories of gene expression by the Arabidopsis bHLH protein MUTE, a potent inducer of stomatal differentiation, and support a notion that MUTE orchestrates a transcriptional cascade leading to the tightly restricted, robust pulse of cell-cycle gene expression, thereby ensuring the single cell division to create functional stomata.
3
Plasticity in stomatal development: what role does MAPK signaling play?
TL;DR: Stomatal precursors in Arabidopsis can be influenced at multiple points in the well-characterized stomatal development pathway by modulation of a core MAPK signaling module, suggesting that this plasticity involves an expanding collection of mitogen activated protein kinase (MAPK) signaling components and putative upstream extracellular ligands.
3
Analysis of differential protein and gene expression in Arabidopsis thaliana depending on iron supply and the abundance of the central iron uptake regulator FIT (Fer-like Iron Deficiency-induced Transcription Factor) and investigations on possible post-translational modifications of FIT
Hans-Jörg Mai
- 01 Jan 2013
TL;DR: Zusammenfassung 7 1 ABSTRACT/ZUSAMMENFASSUNG
3
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