An E. coli cell-free transcription- translation system: modeling gene expression and characterizing CRISPR elements and gene circuits

TL;DR: In this paper, a review of advances in microbial engineering for the production of other potential fuel molecules, using a variety of biosynthetic pathways, is presented, highlighting additional options.

TL;DR: It is suggested that degradation of foreign DNA needs fast translocation, whereas DNA repair uses slower translocation to coordinate RecA loading onto ssDNA.

TL;DR: It is shown that double-stranded DNA encoding χ sites-eight base-pair sequences preferentially bound by the RecBCD recombination machinery-stabilizes linear DNA and greatly enhances the TXTL-based expression and activity of a fluorescent reporter gene, simple regulatory cascades, and T7 bacteriophage particles.

TL;DR: Class 1 CRISPR-Cas systems are characterized by effector modules consisting of multiple subunits consisting ofmultiple subunits and can target both DNA and RNA.

TL;DR: Results demonstrate that polysome analysis is a valid tool to characterize cell-free translation and to identify limiting steps, that dilution of translation factors is a limitation of CFPS, and that CFPS is a useful platform for making novel observations about translation.