An E. coli cell-free transcription- translation system: modeling gene expression and characterizing CRISPR elements and gene circuits

TL;DR: In this paper, a review of advances in microbial engineering for the production of other potential fuel molecules, using a variety of biosynthetic pathways, is presented, highlighting additional options.

TL;DR: It is shown that Cascade efficiently locates target sequences in negatively supercoiled DNA, but only if these are flanked by a protospacer-adjacent motif (PAM), which exclusively involves the crRNA-complementary DNA strand.

TL;DR: Four unique type II-A CRISPR-Cas9 inhibitor proteins encoded by Listeria monocytogenes prophages present tools that can be used to regulate the genome engineering activities of CRISpr-Cas 9.

TL;DR: This paper applied whole-genome transcript arrays to identify promoters that respond to the accumulation of toxic intermediates, and then used these promoters to control accumulation of the intermediate and improve the final titers of a desired product.

TL;DR: By more closely replicating the physiological conditions of the cytoplasm of Escherichia coli, the Cytomim system provides a stable energy supply for protein expression without phosphate accumulation, pH change, exogenous enzyme addition, or the need for expensive high‐energy phosphate compounds.